Detailed information of TA system TA022035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3744350..3745188
• Replicon: chromosome
• Accession: NC_009434
• Organism: Pseudomonas stutzeri A1501

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A4VQ36
• Locus tag: PST_RS17250
• Protein ID: WP_011914487.1
• Coordinates: 3744350..3744862 (-)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A4VQ37
• Locus tag: PST_RS17255
• Protein ID: WP_011914488.1
• Coordinates: 3744859..3745188 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PST_RS17230	3739371..3740018	+	648	WP_011914482.1	hypothetical protein	-
PST_RS17235	3740167..3742281	+	2115	WP_125853257.1	AAA family ATPase	-
PST_RS17240	3742353..3742724	+	372	WP_011914484.1	hypothetical protein	-
PST_RS17245	3742779..3743786	+	1008	WP_041755950.1	WYL domain-containing protein	-
PST_RS17250	3744350..3744862	-	513	WP_011914487.1	type II toxin-antitoxin system YhaV family toxin	Toxin
PST_RS17255	3744859..3745188	-	330	WP_011914488.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
PST_RS17260	3745515..3747734	-	2220	WP_011914489.1	DEAD/DEAH box helicase	-
PST_RS17265	3747709..3749004	-	1296	WP_014597639.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3740101..3759199	19098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 19740.44 Da
• Isoelectric Point: 9.7787

>T22035 WP_011914487.1 NC_009434:c3744862-3744350 [Pseudomonas stutzeri A1501]
MSAGKPEPLVIHGWTVFAHPLFLAQIEVLARQVETLKQKDSVGYVKKNASKRLAAIIKLAFDVIPQDPTRPEYRQGGTLG
DDHKHWFRAKFFQQYRLFFRYHAPSKVIVFAWVNDDDTKRAYQSSDDAYRVFRKMLESGHPPDDWDRLLAEAQTESRRME
ESFARAATVQ

Nucleotide

• Length: 513 bp

>T22035 NC_009434:c3744862-3744350 [Pseudomonas stutzeri A1501]
ATGAGCGCAGGGAAGCCCGAGCCTCTGGTAATTCATGGTTGGACGGTTTTCGCTCATCCACTATTTCTCGCACAGATTGA
GGTACTGGCGCGGCAGGTCGAGACCCTTAAGCAAAAAGACTCAGTCGGATATGTGAAGAAAAACGCCAGTAAACGACTGG
CCGCGATCATTAAGTTGGCATTTGATGTTATTCCTCAAGATCCGACTCGACCGGAATACCGCCAGGGCGGAACGCTCGGT
GACGATCACAAGCACTGGTTTCGCGCCAAGTTCTTTCAGCAGTACCGGCTATTTTTTCGCTACCACGCTCCTAGCAAGGT
TATCGTCTTCGCTTGGGTGAATGACGACGACACAAAACGCGCCTATCAAAGTAGCGACGACGCCTATCGAGTGTTTCGAA
AAATGCTGGAAAGCGGGCACCCGCCGGACGACTGGGATCGGCTCCTGGCTGAGGCGCAGACAGAATCGCGGCGGATGGAG
GAATCATTTGCAAGGGCGGCAACGGTGCAGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12056.53 Da
• Isoelectric Point: 4.5642

>AT22035 WP_011914488.1 NC_009434:c3745188-3744859 [Pseudomonas stutzeri A1501]
MAANFEVESTLTDRYQTTVPETVRRALKLGKRDKIHYTIRPSGEVVLTRVEASGSDDPVLGQFLSFLAADITRHPERLKS
VDTGLVQRLQSLVGDVDVDLDTVLSADDE

Nucleotide

• Length: 330 bp

>AT22035 NC_009434:c3745188-3744859 [Pseudomonas stutzeri A1501]
ATGGCCGCGAACTTCGAAGTCGAATCCACCCTTACCGATCGTTACCAGACCACGGTGCCGGAGACTGTCCGTCGTGCTCT
CAAGTTGGGCAAGCGCGACAAGATTCACTACACCATTCGGCCCAGCGGAGAAGTAGTGCTCACGCGCGTCGAAGCATCCG
GCAGCGATGATCCAGTGCTTGGTCAGTTCCTCAGCTTTCTGGCTGCTGACATCACACGCCATCCCGAGCGGCTGAAATCC
GTGGATACCGGGCTCGTCCAACGGCTCCAATCATTGGTAGGCGATGTCGATGTCGATCTCGATACTGTCCTGTCGGCGGA
CGATGAATGA

Structures

Toxin

• AlphaFold DB: A4VQ36

Antitoxin

• AlphaFold DB: A4VQ37