Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4773103..4773705 Replicon chromosome
Accession NZ_CP085069
Organism Escherichia coli strain P27_L1_05.20

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag LH371_RS22995 Protein ID WP_000897305.1
Coordinates 4773394..4773705 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LH371_RS22990 Protein ID WP_000356397.1
Coordinates 4773103..4773393 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH371_RS22965 (4769029) 4769029..4769931 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
LH371_RS22970 (4769928) 4769928..4770563 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
LH371_RS22975 (4770560) 4770560..4771489 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
LH371_RS22980 (4771819) 4771819..4772061 - 243 WP_001086388.1 protein YiiF -
LH371_RS22985 (4772280) 4772280..4772498 - 219 WP_001295676.1 CopG family transcriptional regulator -
LH371_RS22990 (4773103) 4773103..4773393 - 291 WP_000356397.1 NadS family protein Antitoxin
LH371_RS22995 (4773394) 4773394..4773705 - 312 WP_000897305.1 hypothetical protein Toxin
LH371_RS23000 (4773934) 4773934..4774842 + 909 WP_106474777.1 alpha/beta hydrolase -
LH371_RS23005 (4774906) 4774906..4775847 - 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
LH371_RS23010 (4775892) 4775892..4776329 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
LH371_RS23015 (4776326) 4776326..4777198 - 873 WP_000920762.1 virulence factor BrkB family protein -
LH371_RS23020 (4777192) 4777192..4777791 - 600 WP_001295269.1 glucose-1-phosphatase -
LH371_RS23025 (4777890) 4777890..4778675 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T220348 WP_000897305.1 NZ_CP085069:c4773705-4773394 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T220348 NZ_CP117008:3025649-3025756 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT220348 WP_000356397.1 NZ_CP085069:c4773393-4773103 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT220348 NZ_CP117008:c3025602-3025536 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References