Detailed information of TA system TA220331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1850695..1851197
• Replicon: chromosome
• Accession: NZ_CP085069
• Organism: Escherichia coli strain P27_L1_05.20

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: LH371_RS08800
• Protein ID: WP_000767829.1
• Coordinates: 1850943..1851197 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LH371_RS08795
• Protein ID: WP_001259255.1
• Coordinates: 1850695..1850946 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH371_RS08770 (1845873)	1845873..1846940	-	1068	WP_023356487.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
LH371_RS08775 (1846940)	1846940..1848010	-	1071	WP_000108962.1	histidinol-phosphate transaminase	-
LH371_RS08780 (1848007)	1848007..1849311	-	1305	WP_001369878.1	histidinol dehydrogenase	-
LH371_RS08785 (1849317)	1849317..1850216	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LH371_RS08790 (1850362)	1850362..1850412	-	51	WP_001364200.1	his operon leader peptide	-
LH371_RS08795 (1850695)	1850695..1850946	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LH371_RS08800 (1850943)	1850943..1851197	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LH371_RS08805 (1851280)	1851280..1852104	+	825	WP_000754737.1	SDR family oxidoreductase	-
LH371_RS08810 (1852150)	1852150..1853079	+	930	WP_023356486.1	LysR substrate-binding domain-containing protein	-
LH371_RS08815 (1853294)	1853294..1853356	+	63	WP_010723108.1	membrane protein YoeI	-
LH371_RS08820 (1853346)	1853346..1854704	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
LH371_RS08825 (1854883)	1854883..1855941	+	1059	WP_000492312.1	thiosulfate utilization transporter TsuA/YeeE	-
LH371_RS08830 (1855955)	1855955..1856182	+	228	WP_000985278.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T220331 WP_000767829.1 NZ_CP085069:1850943-1851197 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T220331 NZ_CP117007:64105-64410 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT220331 WP_001259255.1 NZ_CP085069:1850695-1850946 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT220331 NZ_CP117007:63885-64103 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCGGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0