Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1850695..1851197 Replicon chromosome
Accession NZ_CP085069
Organism Escherichia coli strain P27_L1_05.20

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag LH371_RS08800 Protein ID WP_000767829.1
Coordinates 1850943..1851197 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LH371_RS08795 Protein ID WP_001259255.1
Coordinates 1850695..1850946 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH371_RS08770 (1845873) 1845873..1846940 - 1068 WP_023356487.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
LH371_RS08775 (1846940) 1846940..1848010 - 1071 WP_000108962.1 histidinol-phosphate transaminase -
LH371_RS08780 (1848007) 1848007..1849311 - 1305 WP_001369878.1 histidinol dehydrogenase -
LH371_RS08785 (1849317) 1849317..1850216 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
LH371_RS08790 (1850362) 1850362..1850412 - 51 WP_001364200.1 his operon leader peptide -
LH371_RS08795 (1850695) 1850695..1850946 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LH371_RS08800 (1850943) 1850943..1851197 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LH371_RS08805 (1851280) 1851280..1852104 + 825 WP_000754737.1 SDR family oxidoreductase -
LH371_RS08810 (1852150) 1852150..1853079 + 930 WP_023356486.1 LysR substrate-binding domain-containing protein -
LH371_RS08815 (1853294) 1853294..1853356 + 63 WP_010723108.1 membrane protein YoeI -
LH371_RS08820 (1853346) 1853346..1854704 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LH371_RS08825 (1854883) 1854883..1855941 + 1059 WP_000492312.1 thiosulfate utilization transporter TsuA/YeeE -
LH371_RS08830 (1855955) 1855955..1856182 + 228 WP_000985278.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T220331 WP_000767829.1 NZ_CP085069:1850943-1851197 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T220331 NZ_CP117007:64105-64410 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT220331 WP_001259255.1 NZ_CP085069:1850695-1850946 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT220331 NZ_CP117007:63885-64103 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCGGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References