Detailed information of TA system TA220327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 754939..755632
• Replicon: chromosome
• Accession: NZ_CP085069
• Organism: Escherichia coli strain P27_L1_05.20

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LH371_RS03655
• Protein ID: WP_000415584.1
• Coordinates: 754939..755235 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LH371_RS03660
• Protein ID: WP_000650107.1
• Coordinates: 755237..755632 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH371_RS03620 (750027)	750027..750341	-	315	WP_000958598.1	putative quinol monooxygenase	-
LH371_RS03625 (750372)	750372..750953	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LH371_RS03630 (751272)	751272..751604	+	333	WP_023149630.1	DUF2645 family protein	-
LH371_RS03635 (751650)	751650..752999	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LH371_RS03640 (752996)	752996..753655	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
LH371_RS03645 (753807)	753807..754199	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LH371_RS03650 (754252)	754252..754734	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LH371_RS03655 (754939)	754939..755235	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LH371_RS03660 (755237)	755237..755632	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LH371_RS03665 (755765)	755765..757372	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
LH371_RS03670 (757510)	757510..759768	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T220327 WP_000415584.1 NZ_CP085069:754939-755235 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T220327 NZ_CP117006:c4395222-4395049 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT220327 WP_000650107.1 NZ_CP085069:755237-755632 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT220327 NZ_CP117006:c4395546-4395250 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65