220326

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 646826..647625 Replicon chromosome
Accession NZ_CP085069
Organism Escherichia coli strain P27_L1_05.20

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag LH371_RS03130 Protein ID WP_000347267.1
Coordinates 646826..647290 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LH371_RS03135 Protein ID WP_001307405.1
Coordinates 647290..647625 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH371_RS03100 (641827) 641827..642261 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LH371_RS03105 (642279) 642279..643157 - 879 WP_001355782.1 PTS N-acetylgalactosamine transporter subunit IID -
LH371_RS03110 (643147) 643147..643926 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LH371_RS03115 (643937) 643937..644410 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LH371_RS03120 (644433) 644433..645713 - 1281 WP_106474742.1 tagatose-bisphosphate aldolase subunit KbaZ -
LH371_RS03125 (645962) 645962..646771 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LH371_RS03130 (646826) 646826..647290 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LH371_RS03135 (647290) 647290..647625 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LH371_RS03140 (647774) 647774..649345 - 1572 WP_001273741.1 galactarate dehydratase -
LH371_RS03145 (649720) 649720..651054 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LH371_RS03150 (651070) 651070..651840 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T220326 WP_000347267.1 NZ_CP085069:c647290-646826 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T220326 NZ_CP117006:c4212362-4212075 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT220326 WP_001307405.1 NZ_CP085069:c647625-647290 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT220326 NZ_CP117006:c4212088-4211786 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References