Detailed information of TA system TA220319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3611..4323
• Replicon: plasmid unnamed1
• Accession: NZ_CP085065
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8E2KS52
• Locus tag: LH370_RS25760
• Protein ID: WP_016262381.1
• Coordinates: 3611..3913 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LH370_RS25765
• Protein ID: WP_000806445.1
• Coordinates: 3985..4323 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS25740 (LH370_25740)	234..911	-	678	WP_016262377.1	hypothetical protein	-
LH370_RS25745 (LH370_25745)	908..1888	-	981	WP_121372126.1	tail length tape measure protein	-
LH370_RS25750 (LH370_25750)	1892..2659	-	768	WP_016262379.1	hypothetical protein	-
LH370_RS25755 (LH370_25755)	2656..3447	-	792	WP_016262380.1	hypothetical protein	-
LH370_RS25760 (LH370_25760)	3611..3913	+	303	WP_016262381.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LH370_RS25765 (LH370_25765)	3985..4323	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
LH370_RS25770 (LH370_25770)	4380..4739	+	360	WP_097452653.1	transcriptional regulator	-
LH370_RS25775 (LH370_25775)	4801..5538	+	738	WP_183037173.1	hypothetical protein	-
LH370_RS25780 (LH370_25780)	5572..6768	-	1197	WP_097765456.1	phage tail protein	-
LH370_RS25785 (LH370_25785)	6761..7090	-	330	WP_016262385.1	hypothetical protein	-
LH370_RS25790 (LH370_25790)	7422..8075	-	654	WP_021551806.1	hypothetical protein	-
LH370_RS25795 (LH370_25795)	8200..8961	+	762	WP_223363306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94191	94191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11931.72 Da
• Isoelectric Point: 10.0208

>T220319 WP_016262381.1 NZ_CP085065:3611-3913 [Escherichia coli]
MSKKINIKNFRDEWLDDFFEFSIPHKKIPSDIQITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T220319 NZ_CP117006:2769125-2769232 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT220319 WP_000806445.1 NZ_CP085065:3985-4323 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT220319 NZ_CP117006:c2769078-2769012 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT