Detailed information of TA system TA220316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4477289..4478016
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: LH370_RS21560
• Protein ID: WP_001521139.1
• Coordinates: 4477289..4477600 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LH370_RS21565
• Protein ID: WP_000126294.1
• Coordinates: 4477597..4478016 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS21535 (4473224)	4473224..4474933	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
LH370_RS21540 (4474943)	4474943..4475485	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LH370_RS21545 (4475485)	4475485..4476252	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LH370_RS21550 (4476249)	4476249..4476659	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LH370_RS21555 (4476652)	4476652..4477122	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LH370_RS21560 (4477289)	4477289..4477600	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
LH370_RS21565 (4477597)	4477597..4478016	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LH370_RS21570 (4478095)	4478095..4479519	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
LH370_RS21575 (4479528)	4479528..4480985	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LH370_RS21580 (4481245)	4481245..4482255	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
LH370_RS21585 (4482404)	4482404..4482931	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T220316 WP_001521139.1 NZ_CP085063:4477289-4477600 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T220316 NZ_CP117006:2097666-2097769 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT220316 WP_000126294.1 NZ_CP085063:4477597-4478016 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT220316 NZ_CP117006:c2097807-2097536 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A829L2T9