Detailed information of TA system TA220311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3110394..3110996
• Replicon: chromosome
• Accession: NZ_CP085063
• Organism: Escherichia coli strain P31_YH1_02.21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LH370_RS15055
• Protein ID: WP_000897305.1
• Coordinates: 3110685..3110996 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LH370_RS15050
• Protein ID: WP_000356395.1
• Coordinates: 3110394..3110684 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH370_RS15015 (3106017)	3106017..3106919	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LH370_RS15020 (3106916)	3106916..3107551	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LH370_RS15025 (3107548)	3107548..3108477	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LH370_RS15030 (3108659)	3108659..3108901	-	243	WP_021523315.1	hypothetical protein	-
LH370_RS15035 (3109120)	3109120..3109338	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LH370_RS15040 (3109757)	3109757..3110035	-	279	WP_001306650.1	hypothetical protein	-
LH370_RS15045 (3110097)	3110097..3110309	-	213	WP_000197769.1	hypothetical protein	-
LH370_RS15050 (3110394)	3110394..3110684	-	291	WP_000356395.1	NadS family protein	Antitoxin
LH370_RS15055 (3110685)	3110685..3110996	-	312	WP_000897305.1	hypothetical protein	Toxin
LH370_RS15060 (3111225)	3111225..3112133	+	909	WP_001306651.1	alpha/beta hydrolase	-
LH370_RS15065 (3112302)	3112302..3113216	-	915	WP_077634137.1	transposase	-
LH370_RS15070 (3113229)	3113229..3114116	-	888	Protein_2944	hypothetical protein	-
LH370_RS15075 (3114605)	3114605..3115063	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3114605..3115063	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T220311 WP_000897305.1 NZ_CP085063:c3110996-3110685 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T220311 NZ_CP117006:1020145-1020480 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT220311 WP_000356395.1 NZ_CP085063:c3110684-3110394 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT220311 NZ_CP117006:1019897-1020145 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7