220305

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2122080..2122774 Replicon chromosome
Accession NZ_CP085063
Organism Escherichia coli strain P31_YH1_02.21

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag LH370_RS10395 Protein ID WP_001521903.1
Coordinates 2122080..2122478 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LH370_RS10400 Protein ID WP_000554758.1
Coordinates 2122481..2122774 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (2117910) 2117910..2117990 - 81 NuclAT_11 - -
- (2117910) 2117910..2117990 - 81 NuclAT_11 - -
- (2117910) 2117910..2117990 - 81 NuclAT_11 - -
- (2117910) 2117910..2117990 - 81 NuclAT_11 - -
LH370_RS10365 (2117250) 2117250..2118494 - 1245 WP_000189541.1 esterase FrsA -
LH370_RS10370 (2118586) 2118586..2119044 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LH370_RS10375 (2119305) 2119305..2120762 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
LH370_RS10380 (2120819) 2120819..2121171 - 353 Protein_2033 peptide chain release factor H -
LH370_RS10385 (2121167) 2121167..2121373 - 207 Protein_2034 RtcB family protein -
LH370_RS10390 (2121618) 2121618..2122070 - 453 WP_023144376.1 GNAT family N-acetyltransferase -
LH370_RS10395 (2122080) 2122080..2122478 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LH370_RS10400 (2122481) 2122481..2122774 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LH370_RS10405 (2122826) 2122826..2123881 - 1056 WP_001226177.1 DNA polymerase IV -
LH370_RS10410 (2123952) 2123952..2124737 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
LH370_RS10415 (2124709) 2124709..2126421 + 1713 Protein_2040 flagellar biosynthesis protein FlhA -
LH370_RS10420 (2126500) 2126500..2126853 + 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
LH370_RS10425 (2126910) 2126910..2127659 - 750 WP_016233951.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2112017..2122774 10757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T220305 WP_001521903.1 NZ_CP085063:c2122478-2122080 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T220305 NZ_CP117006:219188-219295 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT220305 WP_000554758.1 NZ_CP085063:c2122774-2122481 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT220305 NZ_CP117006:c219130-219076 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References