Detailed information of TA system TA220285

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61801..62065
• Replicon: plasmid unnamed1
• Accession: NZ_CP085062
• Organism: Escherichia coli strain P30_L1_05.20

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: LH369_RS24795
• Protein ID: WP_001387489.1
• Coordinates: 61801..61953 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	62005..62065 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LH369_RS24760 (57366)	57366..57482	+	117	Protein_66	IncI1-type conjugal transfer lipoprotein TraH	-
LH369_RS24765 (57481)	57481..59235	+	1755	Protein_67	DotA/TraY family protein	-
LH369_RS24770 (59306)	59306..59968	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
LH369_RS24775 (60040)	60040..60249	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
LH369_RS24780 (60641)	60641..60817	+	177	WP_001054897.1	hypothetical protein	-
LH369_RS24785 (60882)	60882..61178	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
LH369_RS24790 (61478)	61478..61729	+	252	WP_001291964.1	hypothetical protein	-
LH369_RS24795 (61801)	61801..61953	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (62005)	62005..62065	+	61	NuclAT_0	-	Antitoxin
- (62005)	62005..62065	+	61	NuclAT_0	-	Antitoxin
- (62005)	62005..62065	+	61	NuclAT_0	-	Antitoxin
- (62005)	62005..62065	+	61	NuclAT_0	-	Antitoxin
LH369_RS24800 (62274)	62274..62648	+	375	WP_223349261.1	hypothetical protein	-
LH369_RS24805 (62801)	62801..63958	+	1158	Protein_75	IS1380-like element ISEcp1 family transposase	-
LH369_RS24810 (64014)	64014..64711	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
LH369_RS24815 (64722)	64722..65030	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
LH369_RS24820 (65472)	65472..65759	-	288	WP_000074855.1	conjugal transfer protein TraA	-
LH369_RS24825 (65797)	65797..65955	-	159	Protein_79	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-145	-	1..66866	66866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T220285 WP_001387489.1 NZ_CP085062:c61953-61801 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T220285 NZ_CP117001:1210953-1211330 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT220285 NZ_CP085062:62005-62065 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin