Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3982026..3982753 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LH369_RS19120 Protein ID WP_000550189.1
Coordinates 3982439..3982753 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LH369_RS19115 Protein ID WP_000560266.1
Coordinates 3982026..3982442 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS19105 (3977186) 3977186..3979537 + 2352 WP_000695487.1 alpha-glucosidase -
LH369_RS19110 (3979963) 3979963..3981981 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LH369_RS19115 (3982026) 3982026..3982442 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LH369_RS19120 (3982439) 3982439..3982753 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LH369_RS19125 (3983037) 3983037..3984173 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LH369_RS19130 (3984258) 3984258..3984761 + 504 WP_001333820.1 M48 family metallopeptidase -
LH369_RS19135 (3984838) 3984838..3985530 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LH369_RS19140 (3985609) 3985609..3986595 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T220278 WP_000550189.1 NZ_CP085060:c3982753-3982439 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T220278 NZ_CP117001:c331138-331031 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT220278 WP_000560266.1 NZ_CP085060:c3982442-3982026 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT220278 NZ_CP117001:331186-331252 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References