Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2780744..2781246 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag LH369_RS13535 Protein ID WP_000767831.1
Coordinates 2780744..2780998 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LH369_RS13540 Protein ID WP_001259255.1
Coordinates 2780995..2781246 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS13505 (2775759) 2775759..2775986 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
LH369_RS13510 (2776000) 2776000..2777058 - 1059 WP_000492321.1 thiosulfate utilization transporter TsuA/YeeE -
LH369_RS13515 (2777237) 2777237..2778595 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LH369_RS13520 (2778585) 2778585..2778647 - 63 WP_010723108.1 membrane protein YoeI -
LH369_RS13525 (2778862) 2778862..2779791 - 930 WP_024195433.1 LysR substrate-binding domain-containing protein -
LH369_RS13530 (2779837) 2779837..2780661 - 825 WP_000754737.1 SDR family oxidoreductase -
LH369_RS13535 (2780744) 2780744..2780998 - 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LH369_RS13540 (2780995) 2780995..2781246 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LH369_RS13545 (2781530) 2781530..2781580 + 51 WP_001364200.1 his operon leader peptide -
LH369_RS13550 (2781726) 2781726..2782625 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
LH369_RS13555 (2782631) 2782631..2783935 + 1305 WP_001446927.1 histidinol dehydrogenase -
LH369_RS13560 (2783932) 2783932..2785002 + 1071 WP_000108945.1 histidinol-phosphate transaminase -
LH369_RS13565 (2785002) 2785002..2786069 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T220273 WP_000767831.1 NZ_CP085060:c2780998-2780744 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T220273 NZ_CP116995:c69042-68626 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT220273 WP_001259255.1 NZ_CP085060:c2781246-2780995 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT220273 NZ_CP116995:c69269-69039 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References