Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1785612..1786407 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag LH369_RS08455 Protein ID WP_000854914.1
Coordinates 1786033..1786407 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7UP42
Locus tag LH369_RS08450 Protein ID WP_001280955.1
Coordinates 1785612..1785986 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS08415 (1780967) 1780967..1781872 + 906 WP_023149949.1 diguanylate cyclase regulator RdcB family protein -
LH369_RS08420 (1781869) 1781869..1782939 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
LH369_RS08425 (1783044) 1783044..1783199 - 156 WP_001415943.1 hypothetical protein -
LH369_RS08430 (1783279) 1783279..1784097 + 819 WP_001234682.1 DUF932 domain-containing protein -
LH369_RS08435 (1784188) 1784188..1784673 + 486 WP_000214398.1 antirestriction protein -
LH369_RS08440 (1784689) 1784689..1785165 + 477 WP_001186738.1 RadC family protein -
LH369_RS08445 (1785228) 1785228..1785449 + 222 WP_001220314.1 DUF987 domain-containing protein -
LH369_RS08450 (1785612) 1785612..1785986 + 375 WP_001280955.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LH369_RS08455 (1786033) 1786033..1786407 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
LH369_RS08460 (1786404) 1786404..1786895 + 492 WP_000976857.1 DUF5983 family protein -
LH369_RS08465 (1786907) 1786907..1787104 + 198 WP_032278113.1 DUF957 domain-containing protein -
LH369_RS08470 (1787189) 1787189..1788031 + 843 WP_001280481.1 DUF4942 domain-containing protein -
LH369_RS08480 (1788502) 1788502..1789440 + 939 WP_000351317.1 glyoxylate/hydroxypyruvate reductase GhrA -
LH369_RS08485 (1789495) 1789495..1790232 + 738 WP_000283667.1 zinc-binding phosphatase -
LH369_RS08490 (1790256) 1790256..1790810 + 555 WP_001001921.1 molecular chaperone YcdY -
LH369_RS08495 (1790912) 1790912..1791403 + 492 WP_001300785.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1784689..1797745 13056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T220261 WP_000854914.1 NZ_CP085060:1786033-1786407 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T220261 NZ_CP116994:c1699014-1698616 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13767.59 Da        Isoelectric Point: 6.6248

>AT220261 WP_001280955.1 NZ_CP085060:1785612-1785986 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT220261 NZ_CP116994:c1699241-1699014 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9P0D0

References