Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 846611..847305 Replicon chromosome
Accession NZ_CP085060
Organism Escherichia coli strain P30_L1_05.20

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag LH369_RS04005 Protein ID WP_001263489.1
Coordinates 846907..847305 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LH369_RS04000 Protein ID WP_000554758.1
Coordinates 846611..846904 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LH369_RS03980 (842243) 842243..842740 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
LH369_RS03985 (842964) 842964..844676 - 1713 Protein_769 flagellar biosynthesis protein FlhA -
LH369_RS03990 (844648) 844648..845433 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
LH369_RS03995 (845504) 845504..846559 + 1056 WP_001226164.1 DNA polymerase IV -
LH369_RS04000 (846611) 846611..846904 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LH369_RS04005 (846907) 846907..847305 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LH369_RS04010 (847315) 847315..847767 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
LH369_RS04015 (848085) 848085..848291 + 207 Protein_775 RtcB family protein -
LH369_RS04020 (848287) 848287..848808 + 522 Protein_776 peptide chain release factor H -
LH369_RS04025 (848865) 848865..850322 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
LH369_RS04030 (850583) 850583..851041 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (851637) 851637..851717 + 81 NuclAT_11 - -
- (851637) 851637..851717 + 81 NuclAT_11 - -
- (851637) 851637..851717 + 81 NuclAT_11 - -
- (851637) 851637..851717 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T220259 WP_001263489.1 NZ_CP085060:846907-847305 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T220259 NZ_CP116994:c1226496-1226242 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT220259 WP_000554758.1 NZ_CP085060:846611-846904 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT220259 NZ_CP116994:c1226744-1226493 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References