Detailed information of TA system TA220231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1092723..1093450
• Replicon: chromosome
• Accession: NZ_CP085056
• Organism: Escherichia coli strain P24_WM1_05.20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: LHK10_RS05330
• Protein ID: WP_000547564.1
• Coordinates: 1092723..1093034 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LHK10_RS05335
• Protein ID: WP_000126294.1
• Coordinates: 1093031..1093450 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK10_RS05300 (1087865)	1087865..1089574	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
LHK10_RS05305 (1089584)	1089584..1090126	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
LHK10_RS05310 (1090126)	1090126..1090893	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LHK10_RS05315 (1090890)	1090890..1091300	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
LHK10_RS05320 (1091293)	1091293..1091763	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LHK10_RS05325 (1091788)	1091788..1092549	+	762	WP_001026446.1	hypothetical protein	-
LHK10_RS05330 (1092723)	1092723..1093034	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
LHK10_RS05335 (1093031)	1093031..1093450	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LHK10_RS05340 (1093564)	1093564..1094988	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
LHK10_RS05345 (1094997)	1094997..1096454	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LHK10_RS05350 (1096714)	1096714..1097724	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
LHK10_RS05355 (1097873)	1097873..1098400	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T220231 WP_000547564.1 NZ_CP085056:1092723-1093034 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T220231 NZ_CP116987:1011694-1012029 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT220231 WP_000126294.1 NZ_CP085056:1093031-1093450 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT220231 NZ_CP116987:1011446-1011694 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5