Detailed information of TA system TA220228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 750767..751460
• Replicon: chromosome
• Accession: NZ_CP085056
• Organism: Escherichia coli strain P24_WM1_05.20

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: U9ZN09
• Locus tag: LHK10_RS03665
• Protein ID: WP_000415585.1
• Coordinates: 750767..751063 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LHK10_RS03670
• Protein ID: WP_000650107.1
• Coordinates: 751065..751460 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHK10_RS03630 (745901)	745901..746380	+	480	WP_000065332.1	Hcp family type VI secretion system effector	-
LHK10_RS03635 (746383)	746383..747093	+	711	WP_000834030.1	hypothetical protein	-
LHK10_RS03640 (747100)	747100..747432	+	333	WP_000914690.1	DUF2645 family protein	-
LHK10_RS03645 (747478)	747478..748827	-	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
LHK10_RS03650 (748824)	748824..749483	-	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	-
LHK10_RS03655 (749635)	749635..750027	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LHK10_RS03660 (750080)	750080..750562	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LHK10_RS03665 (750767)	750767..751063	+	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	Toxin
LHK10_RS03670 (751065)	751065..751460	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LHK10_RS03675 (751593)	751593..753200	+	1608	WP_001375094.1	ABC transporter substrate-binding protein	-
LHK10_RS03680 (753338)	753338..755596	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11261.99 Da
• Isoelectric Point: 8.9070

>T220228 WP_000415585.1 NZ_CP085056:750767-751063 [Escherichia coli]
MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T220228 NZ_CP116987:304979-305506 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT220228 WP_000650107.1 NZ_CP085056:751065-751460 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT220228 NZ_CP116987:304706-304972 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: U9ZN09

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65