Detailed information of TA system TA220170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 60766..61417
• Replicon: plasmid pCEP14_03
• Accession: NZ_CP084924
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: LG653_RS20185
• Protein ID: WP_004668307.1
• Coordinates: 61061..61417 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LG653_RS20180
• Protein ID: WP_001140620.1
• Coordinates: 60766..61068 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS20160 (LG653_20160)	57632..58336	-	705	Protein_48	IS6-like element IS1006 family transposase	-
LG653_RS20165 (LG653_20165)	58496..59800	-	1305	WP_068558395.1	Y-family DNA polymerase	-
LG653_RS20170 (LG653_20170)	59816..60394	-	579	WP_068520013.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LG653_RS20175 (LG653_20175)	60566..60724	+	159	Protein_51	IS6 family transposase	-
LG653_RS20180 (LG653_20180)	60766..61068	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
LG653_RS20185 (LG653_20185)	61061..61417	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG653_RS20190 (LG653_20190)	62216..62509	-	294	WP_038349496.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LG653_RS20195 (LG653_20195)	62499..62745	-	247	Protein_55	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LG653_RS20200 (LG653_20200)	63063..63590	+	528	Protein_56	DUF4263 domain-containing protein	-
LG653_RS20205 (LG653_20205)	63683..64237	+	555	WP_226789440.1	hypothetical protein	-
LG653_RS20215 (LG653_20215)	65420..65566	-	147	Protein_59	resolvase	-
LG653_RS20220 (LG653_20220)	65747..66274	-	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89672	89672
	-	inside	IScluster/Tn	-	-	57632..67550	9918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T220170 WP_004668307.1 NZ_CP084924:c61417-61061 [Acinetobacter pittii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T220170 NZ_CP116972:2255-2533 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT220170 WP_001140620.1 NZ_CP084924:c61068-60766 [Acinetobacter pittii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT220170 NZ_CP116972:1986-2255 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: N9NM16