Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 15932..16583 Replicon plasmid pCEP14_03
Accession NZ_CP084924
Organism Acinetobacter pittii strain CEP14

Toxin (Protein)


Gene name higB Uniprot ID N9NM16
Locus tag LG653_RS19995 Protein ID WP_004668307.1
Coordinates 16227..16583 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LG653_RS19990 Protein ID WP_001140620.1
Coordinates 15932..16234 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LG653_RS19970 (LG653_19970) 12799..13503 - 705 Protein_10 IS6-like element IS1006 family transposase -
LG653_RS19975 (LG653_19975) 13663..14967 - 1305 WP_068558395.1 Y-family DNA polymerase -
LG653_RS19980 (LG653_19980) 14983..15561 - 579 WP_068520013.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
LG653_RS19985 (LG653_19985) 15733..15891 + 159 Protein_13 IS6 family transposase -
LG653_RS19990 (LG653_19990) 15932..16234 - 303 WP_001140620.1 XRE family transcriptional regulator Antitoxin
LG653_RS19995 (LG653_19995) 16227..16583 - 357 WP_004668307.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LG653_RS20000 (LG653_20000) 17382..17675 - 294 WP_038349496.1 type II toxin-antitoxin system RelE/ParE family toxin -
LG653_RS20005 (LG653_20005) 17665..17910 - 246 WP_000246756.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
LG653_RS20010 (LG653_20010) 18116..18754 + 639 WP_071211298.1 DUF4263 domain-containing protein -
LG653_RS20015 (LG653_20015) 18847..19401 + 555 WP_226789440.1 hypothetical protein -
LG653_RS20025 (LG653_20025) 20584..20730 - 147 Protein_21 resolvase -
LG653_RS20030 (LG653_20030) 20911..21438 - 528 WP_000537572.1 plasmid pRiA4b ORF-3 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..89672 89672
- inside IScluster/Tn - - 12799..22712 9913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

HigA2-like, helix-turn-helix domain

(14-89)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13610.64 Da        Isoelectric Point: 7.1651

>T220167 WP_004668307.1 NZ_CP084924:c16583-16227 [Acinetobacter pittii]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Download         Length: 357 bp

>T220167 NZ_CP116971:c90884-90735 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11227.02 Da        Isoelectric Point: 6.4759

>AT220167 WP_001140620.1 NZ_CP084924:c16234-15932 [Acinetobacter pittii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Download         Length: 303 bp

>AT220167 NZ_CP116971:90928-90989 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N9NM16

Antitoxin

Source ID Structure

References