Detailed information of TA system TA220166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 55673..56324
• Replicon: plasmid pCEP14_02
• Accession: NZ_CP084923
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A062G190
• Locus tag: LG653_RS19890
• Protein ID: WP_005065055.1
• Coordinates: 55673..56017 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LG653_RS19895
• Protein ID: WP_005065057.1
• Coordinates: 56022..56324 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS19870 (LG653_19870)	51536..52249	+	714	WP_005065071.1	hypothetical protein	-
LG653_RS19875 (LG653_19875)	52947..53876	+	930	WP_005065049.1	replication initiation protein RepM	-
LG653_RS19880 (LG653_19880)	53955..54485	+	531	WP_005065050.1	plasmid replication DNA-binding protein	-
LG653_RS19885 (LG653_19885)	54835..55152	+	318	WP_000900860.1	hypothetical protein	-
LG653_RS19890 (LG653_19890)	55673..56017	+	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG653_RS19895 (LG653_19895)	56022..56324	+	303	WP_005065057.1	XRE family transcriptional regulator	Antitoxin
LG653_RS19900 (LG653_19900)	56416..56817	+	402	WP_005065059.1	hypothetical protein	-
LG653_RS19905 (LG653_19905)	56869..57540	+	672	Protein_66	HEPN domain-containing protein	-
LG653_RS19910 (LG653_19910)	57631..57942	-	312	WP_005065064.1	BrnA antitoxin family protein	-
LG653_RS19915 (LG653_19915)	57929..58216	-	288	WP_002124950.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58452	58452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13143.23 Da
• Isoelectric Point: 6.4651

>T220166 WP_005065055.1 NZ_CP084923:55673-56017 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSGKKRFYTEMLAIADKQYELHLSTLGE

Nucleotide

• Length: 345 bp

>T220166 NZ_CP116971:c87857-87579 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 5.8961

>AT220166 WP_005065057.1 NZ_CP084923:56022-56324 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT220166 NZ_CP116971:c88126-87857 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACAGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A062G190