Detailed information of TA system TA220164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 44515..45166
• Replicon: plasmid pCEP14_02
• Accession: NZ_CP084923
• Organism: Acinetobacter pittii strain CEP14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A062G190
• Locus tag: LG653_RS19825
• Protein ID: WP_005065055.1
• Coordinates: 44515..44859 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LG653_RS19830
• Protein ID: WP_005065057.1
• Coordinates: 44864..45166 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LG653_RS19805 (LG653_19805)	40381..41094	+	714	WP_005065071.1	hypothetical protein	-
LG653_RS19810 (LG653_19810)	41789..42718	+	930	WP_005065049.1	replication initiation protein RepM	-
LG653_RS19815 (LG653_19815)	42797..43327	+	531	WP_005065050.1	plasmid replication DNA-binding protein	-
LG653_RS19820 (LG653_19820)	43677..43994	+	318	WP_000900860.1	hypothetical protein	-
LG653_RS19825 (LG653_19825)	44515..44859	+	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LG653_RS19830 (LG653_19830)	44864..45166	+	303	WP_005065057.1	XRE family transcriptional regulator	Antitoxin
LG653_RS19835 (LG653_19835)	45258..45659	+	402	WP_005065059.1	hypothetical protein	-
LG653_RS19840 (LG653_19840)	45711..46385	+	675	WP_005065062.1	HEPN domain-containing protein	-
LG653_RS19845 (LG653_19845)	46476..46787	-	312	WP_005065064.1	BrnA antitoxin family protein	-
LG653_RS19850 (LG653_19850)	46774..47061	-	288	WP_002124950.1	BrnT family toxin	-
LG653_RS19855 (LG653_19855)	47574..49061	+	1488	WP_226789433.1	SulP family inorganic anion transporter	-
LG653_RS19860 (LG653_19860)	49074..49925	+	852	WP_005065068.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58452	58452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13143.23 Da
• Isoelectric Point: 6.4651

>T220164 WP_005065055.1 NZ_CP084923:44515-44859 [Acinetobacter pittii]
MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSGKKRFYTEMLAIADKQYELHLSTLGE

Nucleotide

• Length: 345 bp

>T220164 NZ_CP116970:c4329193-4328651 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTAATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 5.8961

>AT220164 WP_005065057.1 NZ_CP084923:44864-45166 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT220164 NZ_CP116970:c4329488-4329177 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A062G190