Detailed information of TA system TA220141

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 126609..126842
• Replicon: plasmid pEC22-OXA-1
• Accession: NZ_CP084902
• Organism: Escherichia coli strain EC20

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LHL15_RS25430
• Protein ID: WP_001372321.1
• Coordinates: 126609..126734 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	126811..126842 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHL15_RS25390 (121983)	121983..122672	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
LHL15_RS25395 (122859)	122859..123242	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LHL15_RS25400 (123563)	123563..124165	+	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
LHL15_RS25405 (124462)	124462..125283	-	822	WP_001234426.1	DUF932 domain-containing protein	-
LHL15_RS25410 (125401)	125401..125688	-	288	WP_000107535.1	hypothetical protein	-
LHL15_RS25415 (125713)	125713..125919	-	207	WP_000547968.1	hypothetical protein	-
LHL15_RS25420 (125989)	125989..126161	+	173	Protein_150	hypothetical protein	-
LHL15_RS25425 (126159)	126159..126389	-	231	WP_071886920.1	hypothetical protein	-
LHL15_RS25430 (126609)	126609..126734	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LHL15_RS25435 (126676)	126676..126825	-	150	Protein_153	plasmid maintenance protein Mok	-
- (126811)	126811..126842	-	32	NuclAT_1	-	Antitoxin
- (126811)	126811..126842	-	32	NuclAT_1	-	Antitoxin
- (126811)	126811..126842	-	32	NuclAT_1	-	Antitoxin
- (126811)	126811..126842	-	32	NuclAT_1	-	Antitoxin
- (128284)	128284..128481	-	198	NuclAT_0	-	-
- (128284)	128284..128481	-	198	NuclAT_0	-	-
- (128284)	128284..128481	-	198	NuclAT_0	-	-
- (128284)	128284..128481	-	198	NuclAT_0	-	-
LHL15_RS25445 (128293)	128293..128481	+	189	WP_001299721.1	hypothetical protein	-
LHL15_RS25450 (128450)	128450..129212	-	763	Protein_156	plasmid SOS inhibition protein A	-
LHL15_RS25455 (129209)	129209..129643	-	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
LHL15_RS25460 (129698)	129698..129895	-	198	Protein_158	hypothetical protein	-
LHL15_RS25465 (129923)	129923..130156	-	234	WP_000005990.1	DUF905 family protein	-
LHL15_RS25470 (130224)	130224..130763	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
LHL15_RS25475 (130789)	130789..130995	-	207	WP_000275856.1	hypothetical protein	-
LHL15_RS25480 (131235)	131235..131486	-	252	WP_071579736.1	hypothetical protein	-
LHL15_RS25485 (131405)	131405..131611	-	207	WP_001774176.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..169208	169208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T220141 WP_001372321.1 NZ_CP084902:c126734-126609 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T220141 NZ_CP116962:c1257346-1257002 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCGGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGAAGGTCAGTTTAATTATTCTAATGTGGTTACCCATTACAATTTAAAGAT
GGGACAGGAAACTTATTTAAAATAA

Antitoxin

Nucleotide

• Length: 32 bp

>AT220141 NZ_CP084902:c126842-126811 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin