Detailed information of TA system TA220137

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86533..86787
• Replicon: plasmid pEC22-OXA-1
• Accession: NZ_CP084902
• Organism: Escherichia coli strain EC20

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LHL15_RS25185
• Protein ID: WP_001312851.1
• Coordinates: 86533..86682 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	86726..86787 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHL15_RS25155 (81780)	81780..82691	-	912	WP_000440183.1	carbamate kinase	-
LHL15_RS25160 (82702)	82702..83922	-	1221	WP_000410951.1	arginine deiminase	-
LHL15_RS25165 (84629)	84629..85243	+	615	Protein_99	VENN motif pre-toxin domain-containing protein	-
LHL15_RS25170 (85243)	85243..85689	-	447	Protein_100	plasmid replication initiator RepA	-
LHL15_RS25175 (85682)	85682..85756	-	75	WP_032336874.1	RepA leader peptide Tap	-
LHL15_RS25180 (85992)	85992..86249	-	258	WP_000083833.1	replication regulatory protein RepA	-
LHL15_RS25185 (86533)	86533..86682	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (86726)	86726..86787	+	62	NuclAT_2	-	Antitoxin
- (86726)	86726..86787	+	62	NuclAT_2	-	Antitoxin
- (86726)	86726..86787	+	62	NuclAT_2	-	Antitoxin
- (86726)	86726..86787	+	62	NuclAT_2	-	Antitoxin
LHL15_RS25190 (86926)	86926..87108	-	183	WP_000968309.1	hypothetical protein	-
LHL15_RS25195 (87209)	87209..87825	+	617	Protein_105	IS1-like element IS1A family transposase	-
LHL15_RS25200 (87863)	87863..89434	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
LHL15_RS25205 (89454)	89454..89801	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
LHL15_RS25210 (89801)	89801..90478	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
LHL15_RS25215 (90533)	90533..90622	+	90	Protein_109	IS1 family transposase	-
LHL15_RS25220 (90923)	90923..91135	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..169208	169208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T220137 WP_001312851.1 NZ_CP084902:c86682-86533 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T220137 NZ_CP116962:276730-277071 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
TTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT220137 NZ_CP084902:86726-86787 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin