Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 3845550..3846091 | Replicon | chromosome |
| Accession | NZ_CP084900 | ||
| Organism | Escherichia coli strain EC20 | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | S1FHQ9 |
| Locus tag | LHL15_RS18695 | Protein ID | WP_000615979.1 |
| Coordinates | 3845813..3846091 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | S1EWQ0 |
| Locus tag | LHL15_RS18690 | Protein ID | WP_000729704.1 |
| Coordinates | 3845550..3845810 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LHL15_RS18670 (3841225) | 3841225..3842010 | - | 786 | WP_000207564.1 | putative lateral flagellar export/assembly protein LafU | - |
| LHL15_RS18675 (3841982) | 3841982..3843694 | + | 1713 | Protein_3659 | flagellar biosynthesis protein FlhA | - |
| LHL15_RS18680 (3843918) | 3843918..3844415 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
| LHL15_RS18685 (3844591) | 3844591..3845364 | - | 774 | WP_123297815.1 | C40 family peptidase | - |
| LHL15_RS18690 (3845550) | 3845550..3845810 | + | 261 | WP_000729704.1 | type II toxin-antitoxin system antitoxin DinJ | Antitoxin |
| LHL15_RS18695 (3845813) | 3845813..3846091 | + | 279 | WP_000615979.1 | type II toxin-antitoxin system mRNA interferase toxin YafQ | Toxin |
| LHL15_RS18700 (3846247) | 3846247..3846987 | + | 741 | WP_001225679.1 | peptidoglycan meso-diaminopimelic acid protein amidase | - |
| LHL15_RS18705 (3846958) | 3846958..3847725 | - | 768 | WP_000333379.1 | class II glutamine amidotransferase | - |
| LHL15_RS18710 (3847931) | 3847931..3848509 | - | 579 | WP_000284050.1 | D-sedoheptulose 7-phosphate isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10830.62 Da Isoelectric Point: 10.0702
>T220133 WP_000615979.1 NZ_CP084900:3845813-3846091 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T220133 NZ_CP116958:c1532507-1531719 [Streptococcus pasteurianus]
ATGAGATTGGAAGAATTTAGTGAGGTAGAGTTTCAGAAGGCCTTACAGCGAACCATTCGGGCTTTAACTCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCCATTTTACTTGGAGGTCAGAGCGGAGCAGGTAAGACAACGATACATCGTATCAAGCAAA
AAGAATTTCAAGGCAATATCATTATCATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCTTGCAAGAA
AAGTATGGCAAAGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAGATGGTAGAACATCTGGTTGATGAACTCAGTAA
ACGTGGTTATCATTTGTTAATTGAAGGGACTTTGCGTACCACTCAAGTTCCTCGTCAGACTGCTCAATTATTAACTTCTA
AAGGTTACCAAGTTTCTTTAGCTATTATTGGTACCAAACCAGAACTGTCCTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACGCAGGCCAGAGCAACACCCAAAGACCACCATGATGGGATTGTAGAGAACTTGGTTGATAA
CCTAAGAGAGTTAGAAAGAGAGCAACTCTTTGACCAGATTCAGATTTATCAAAGAGATAGGGCTTGTATCTATGATTCTG
AAACGGATGAAGGTTCCGCAGCAGAAGTTCTACAAGACTGCCTCTTTGGAAAATGTAGTAAGGTTGAGGAAGAGATGATG
AAGTTAGGTCGGGAACGGTTAGTTAAACTGAATAATAAGAATTTGCTGGAGAGTAACTATGGAATTTAA
ATGAGATTGGAAGAATTTAGTGAGGTAGAGTTTCAGAAGGCCTTACAGCGAACCATTCGGGCTTTAACTCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCCATTTTACTTGGAGGTCAGAGCGGAGCAGGTAAGACAACGATACATCGTATCAAGCAAA
AAGAATTTCAAGGCAATATCATTATCATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCTTGCAAGAA
AAGTATGGCAAAGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAGATGGTAGAACATCTGGTTGATGAACTCAGTAA
ACGTGGTTATCATTTGTTAATTGAAGGGACTTTGCGTACCACTCAAGTTCCTCGTCAGACTGCTCAATTATTAACTTCTA
AAGGTTACCAAGTTTCTTTAGCTATTATTGGTACCAAACCAGAACTGTCCTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACGCAGGCCAGAGCAACACCCAAAGACCACCATGATGGGATTGTAGAGAACTTGGTTGATAA
CCTAAGAGAGTTAGAAAGAGAGCAACTCTTTGACCAGATTCAGATTTATCAAAGAGATAGGGCTTGTATCTATGATTCTG
AAACGGATGAAGGTTCCGCAGCAGAAGTTCTACAAGACTGCCTCTTTGGAAAATGTAGTAAGGTTGAGGAAGAGATGATG
AAGTTAGGTCGGGAACGGTTAGTTAAACTGAATAATAAGAATTTGCTGGAGAGTAACTATGGAATTTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9391.77 Da Isoelectric Point: 5.0758
>AT220133 WP_000729704.1 NZ_CP084900:3845550-3845810 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT220133 NZ_CP116958:c1532983-1532507 [Streptococcus pasteurianus]
ATGATTGGAGACAATATTAAATCACTACGCCGAACACACGATTTAACACAACCAGAATTTGCGAAAATGGTCGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGAACCAGCACCGTTTCAACAGAACTCATTGACCGCATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGCTAACACCTGTTGAAGATTACCAACTGACTTTAAAAATAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCTCAACTCTATCGATATCAGGATAGTCAAGGTATTGCTTGTGATGATGA
AACAAATCCTTGGATTTTGATGAGTGATGATTTGGCTGAATTGATCAATACGAAAATATACCTTGTCGATACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGACGGTATCGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA
ATGATTGGAGACAATATTAAATCACTACGCCGAACACACGATTTAACACAACCAGAATTTGCGAAAATGGTCGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGAACCAGCACCGTTTCAACAGAACTCATTGACCGCATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGCTAACACCTGTTGAAGATTACCAACTGACTTTAAAAATAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCTCAACTCTATCGATATCAGGATAGTCAAGGTATTGCTTGTGATGATGA
AACAAATCCTTGGATTTTGATGAGTGATGATTTGGCTGAATTGATCAATACGAAAATATACCTTGTCGATACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGACGGTATCGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1FHQ9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 4ML0 | |
| AlphaFold DB | A0A0E0Y6Z6 |