Detailed information of TA system TA220132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3839353..3840047
• Replicon: chromosome
• Accession: NZ_CP084900
• Organism: Escherichia coli strain EC20

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: LHL15_RS18655
• Protein ID: WP_001263493.1
• Coordinates: 3839353..3839751 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LHL15_RS18660
• Protein ID: WP_000554757.1
• Coordinates: 3839754..3840047 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3835013)	3835013..3835093	-	81	NuclAT_11	-	-
- (3835013)	3835013..3835093	-	81	NuclAT_11	-	-
- (3835013)	3835013..3835093	-	81	NuclAT_11	-	-
- (3835013)	3835013..3835093	-	81	NuclAT_11	-	-
LHL15_RS18625 (3834353)	3834353..3835597	-	1245	WP_000189541.1	esterase FrsA	-
LHL15_RS18630 (3835689)	3835689..3836147	-	459	WP_061349133.1	xanthine phosphoribosyltransferase	-
LHL15_RS18635 (3836408)	3836408..3837865	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
LHL15_RS18640 (3837922)	3837922..3838443	-	522	Protein_3652	peptide chain release factor H	-
LHL15_RS18645 (3838442)	3838442..3838645	-	204	Protein_3653	RtcB family protein	-
LHL15_RS18650 (3838891)	3838891..3839343	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
LHL15_RS18655 (3839353)	3839353..3839751	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LHL15_RS18660 (3839754)	3839754..3840047	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LHL15_RS18665 (3840099)	3840099..3841154	-	1056	WP_001226164.1	DNA polymerase IV	-
LHL15_RS18670 (3841225)	3841225..3842010	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
LHL15_RS18675 (3841982)	3841982..3843694	+	1713	Protein_3659	flagellar biosynthesis protein FlhA	-
LHL15_RS18680 (3843918)	3843918..3844415	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T220132 WP_001263493.1 NZ_CP084900:c3839751-3839353 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T220132 NZ_CP116958:c1045618-1045361 [Streptococcus pasteurianus]
ATGATTAAAGCATGGTCTGACGATGCGTGGAACGACTATATGTATTGGTATAACCAAGGAAATAAAGCGACAATTAAAAA
AATTAATCGCCTAATCAAGGAGATTGACCGCACTCCTTTTACTGGCATTGGTAAACCTGAACCACTTAAACACGATTTAT
CTGGCAAATGGTCACGACGTATAACCGATGAACATCGTTTAATTTACAGTGTTGATGAGACTACTATTTACTTTTATTCT
TGCAAAGACCATTATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT220132 WP_000554757.1 NZ_CP084900:c3840047-3839754 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT220132 NZ_CP116958:c1045877-1045611 [Streptococcus pasteurianus]
ATGGAAGCTGTTGCATATTCAAATTTTAGACAAAATTTAAAATATTATATGAAACAAGTTAACGATGACGCTGAACCACT
TATTGTGACTTCTAAAAATATTGATGATACTGTCGTAGTCCTTTCAAAACGTGACTATGATAGCATGCAAGAAACATTTC
GCATTATGTCTAATAACTACCTAATGAGTAAAATTAAACGCGGCGATGAACAATTCAAGGCAGCTAATTTTCAGACACAT
GAATTGCTCGAGGTAGATGATGATTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6