Detailed information of TA system TA220114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 750497..751190
• Replicon: chromosome
• Accession: NZ_CP084900
• Organism: Escherichia coli strain EC20

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LHL15_RS03620
• Protein ID: WP_000415584.1
• Coordinates: 750497..750793 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LHL15_RS03625
• Protein ID: WP_000650107.1
• Coordinates: 750795..751190 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHL15_RS03585 (745585)	745585..745899	-	315	WP_000958598.1	putative quinol monooxygenase	-
LHL15_RS03590 (745930)	745930..746511	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LHL15_RS03595 (746830)	746830..747162	+	333	WP_000917684.1	DUF2645 family protein	-
LHL15_RS03600 (747208)	747208..748557	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LHL15_RS03605 (748554)	748554..749213	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
LHL15_RS03610 (749365)	749365..749757	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LHL15_RS03615 (749810)	749810..750292	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LHL15_RS03620 (750497)	750497..750793	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LHL15_RS03625 (750795)	750795..751190	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LHL15_RS03630 (751323)	751323..752930	+	1608	WP_064221462.1	ABC transporter substrate-binding protein	-
LHL15_RS03635 (753068)	753068..755326	+	2259	WP_104763973.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T220114 WP_000415584.1 NZ_CP084900:750497-750793 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T220114 NZ_CP116931:2929107-2929601 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT220114 WP_000650107.1 NZ_CP084900:750795-751190 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT220114 NZ_CP116931:2928817-2929110 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65