Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 686025..686752 Replicon chromosome
Accession NZ_CP084900
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LHL15_RS03315 Protein ID WP_000550189.1
Coordinates 686025..686339 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LHL15_RS03320 Protein ID WP_000560266.1
Coordinates 686336..686752 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHL15_RS03295 (682183) 682183..683169 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
LHL15_RS03300 (683248) 683248..683940 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LHL15_RS03305 (684017) 684017..684520 - 504 WP_001300832.1 M48 family metallopeptidase -
LHL15_RS03310 (684605) 684605..685741 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LHL15_RS03315 (686025) 686025..686339 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LHL15_RS03320 (686336) 686336..686752 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LHL15_RS03325 (686797) 686797..688815 - 2019 WP_064221460.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LHL15_RS03330 (689141) 689141..691492 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T220113 WP_000550189.1 NZ_CP084900:686025-686339 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T220113 NZ_CP116931:2801065-2801172 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT220113 WP_000560266.1 NZ_CP084900:686336-686752 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT220113 NZ_CP116931:c2801015-2800952 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References