Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 637498..638297 Replicon chromosome
Accession NZ_CP084900
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag LHL15_RS03070 Protein ID WP_000347251.1
Coordinates 637498..637962 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag LHL15_RS03075 Protein ID WP_001308975.1
Coordinates 637962..638297 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHL15_RS03040 (632499) 632499..632933 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LHL15_RS03045 (632951) 632951..633829 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
LHL15_RS03050 (633819) 633819..634598 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LHL15_RS03055 (634609) 634609..635082 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LHL15_RS03060 (635105) 635105..636385 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LHL15_RS03065 (636634) 636634..637443 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LHL15_RS03070 (637498) 637498..637962 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LHL15_RS03075 (637962) 637962..638297 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LHL15_RS03080 (638446) 638446..640017 - 1572 WP_064221457.1 galactarate dehydratase -
LHL15_RS03085 (640392) 640392..641726 + 1335 WP_001701554.1 galactarate/glucarate/glycerate transporter GarP -
LHL15_RS03090 (641742) 641742..642512 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T220112 WP_000347251.1 NZ_CP084900:c637962-637498 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T220112 NZ_CP116931:2800530-2800637 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT220112 WP_001308975.1 NZ_CP084900:c638297-637962 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT220112 NZ_CP116931:c2800483-2800417 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References