220095

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2094178..2094680 Replicon chromosome
Accession NZ_CP084899
Organism Escherichia coli strain W3110-P

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag LHV60_RS10435 Protein ID WP_000767829.1
Coordinates 2094178..2094432 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LHV60_RS10440 Protein ID WP_001259255.1
Coordinates 2094429..2094680 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV60_RS10405 (2089193) 2089193..2089420 - 228 WP_000234896.1 sulfurtransferase TusA -
LHV60_RS10410 (2089434) 2089434..2090492 - 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
LHV60_RS10415 (2090671) 2090671..2092029 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LHV60_RS10420 (2092019) 2092019..2092081 - 63 WP_010723108.1 membrane protein YoeI -
LHV60_RS10425 (2092296) 2092296..2093225 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
LHV60_RS10430 (2093271) 2093271..2094095 - 825 WP_000754737.1 SDR family oxidoreductase -
LHV60_RS10435 (2094178) 2094178..2094432 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LHV60_RS10440 (2094429) 2094429..2094680 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LHV60_RS10445 (2094963) 2094963..2095013 + 51 WP_001364200.1 his operon leader peptide -
LHV60_RS10450 (2095159) 2095159..2096058 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
LHV60_RS10455 (2096064) 2096064..2097368 + 1305 WP_000009594.1 histidinol dehydrogenase -
LHV60_RS10460 (2097365) 2097365..2098435 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
LHV60_RS10465 (2098435) 2098435..2099502 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T220095 WP_000767829.1 NZ_CP084899:c2094432-2094178 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T220095 NZ_CP116929:c3860404-3860117 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT220095 WP_001259255.1 NZ_CP084899:c2094680-2094429 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT220095 NZ_CP116929:c3860130-3859828 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References