Detailed information of TA system TA022008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 953126..953719
• Replicon: chromosome
• Accession: NC_009142
• Organism: Saccharopolyspora erythraea NRRL 2338

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2A9IZT4
• Locus tag: SACE_RS04205
• Protein ID: WP_029621452.1
• Coordinates: 953126..953407 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SACE_RS04210
• Protein ID: WP_011873183.1
• Coordinates: 953420..953719 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACE_RS04185	948486..949517	+	1032	WP_009944211.1	ABC transporter ATP-binding protein	-
SACE_RS04190	949510..950511	+	1002	WP_009944207.1	dipeptide ABC transporter ATP-binding protein	-
SACE_RS04195	950763..952241	-	1479	WP_009944204.1	peptidase S10	-
SACE_RS04200	952326..953072	+	747	WP_037303372.1	uracil-DNA glycosylase	-
SACE_RS04205	953126..953407	+	282	WP_029621452.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SACE_RS04210	953420..953719	+	300	WP_011873183.1	HigA family addiction module antidote protein	Antitoxin
SACE_RS04215	954083..954889	-	807	WP_009944200.1	metalloregulator ArsR/SmtB family transcription factor	-
SACE_RS04220	955396..956718	+	1323	WP_009944198.1	NAD(P)/FAD-dependent oxidoreductase	-
SACE_RS04225	957046..958200	+	1155	WP_009944195.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11019.53 Da
• Isoelectric Point: 10.2071

>T22008 WP_029621452.1 NC_009142:953126-953407 [Saccharopolyspora erythraea NRRL 2338]
MLRSFADKETERVWQRRRSRMLDPKVQRAALRKLAILDAAETVEDLRVPPGNQLEKLNGDRVGQYSIRINQQWRIRFEWT
SAGPENVEIVDYH

Nucleotide

• Length: 282 bp

>T22008 NC_009142:953126-953407 [Saccharopolyspora erythraea NRRL 2338]
ATGCTGCGATCTTTCGCTGACAAGGAGACCGAGCGTGTATGGCAACGACGACGTTCGCGGATGCTCGATCCGAAGGTTCA
GCGGGCGGCTCTGCGTAAGCTCGCCATCCTTGATGCGGCGGAGACCGTGGAAGACCTGCGAGTCCCACCTGGAAACCAAC
TGGAGAAGCTGAACGGAGATCGGGTCGGCCAGTACAGCATCCGGATCAACCAGCAGTGGCGGATCCGCTTTGAGTGGACA
TCAGCCGGGCCCGAGAACGTCGAGATCGTGGACTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11207.81 Da
• Isoelectric Point: 5.6335

>AT22008 WP_011873183.1 NC_009142:953420-953719 [Saccharopolyspora erythraea NRRL 2338]
MSDECVMPPVHPGEVLAEEYLEPLGVTQHRLAVAIGVPPRRINEIVHGKRGISADTALRLARFFGTSERFWLNLQSRYDL
ERERDALADTLDRIQPLSV

Nucleotide

• Length: 300 bp

>AT22008 NC_009142:953420-953719 [Saccharopolyspora erythraea NRRL 2338]
ATGTCTGACGAGTGCGTCATGCCTCCGGTTCATCCCGGGGAAGTCCTGGCCGAGGAGTACTTGGAGCCGCTCGGCGTCAC
ACAGCACCGGTTGGCCGTGGCGATCGGCGTTCCGCCGCGCCGGATCAACGAGATCGTGCACGGCAAGCGTGGGATCTCGG
CCGACACGGCGCTGCGGCTCGCGCGCTTCTTCGGCACCTCCGAGAGGTTCTGGCTCAATCTTCAGTCGCGCTACGACTTG
GAACGTGAGAGGGACGCCCTCGCTGACACGTTGGACCGGATCCAACCGCTGTCCGTGTGA

Structures

Toxin

• AlphaFold DB: A0A2A9IZT4