Detailed information of TA system TA220065

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 4589806..4590218
• Replicon: chromosome
• Accession: NZ_CP084898
• Organism: Escherichia coli strain gw25

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LHV65_RS22295
• Protein ID: WP_000132601.1
• Coordinates: 4589806..4590147 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4590142..4590218 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV65_RS22285 (4587219)	4587219..4588265	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
LHV65_RS22290 (4588265)	4588265..4589644	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LHV65_RS22295 (4589806)	4589806..4590147	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
- (4590142)	4590142..4590218	+	77	NuclAT_14	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_14	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_14	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_14	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_15	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_15	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_15	-	Antitoxin
- (4590142)	4590142..4590218	+	77	NuclAT_15	-	Antitoxin
LHV65_RS22300 (4590375)	4590375..4591769	-	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
LHV65_RS22305 (4591766)	4591766..4593355	-	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T220065 WP_000132601.1 NZ_CP084898:c4590147-4589806 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T220065 NZ_CP116925:c1817250-1816876 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT220065 NZ_CP084898:4590142-4590218 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin