Detailed information of TA system TA220057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3173926..3174619
• Replicon: chromosome
• Accession: NZ_CP084898
• Organism: Escherichia coli strain gw25

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LHV65_RS15490
• Protein ID: WP_000415584.1
• Coordinates: 3174323..3174619 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LHV65_RS15485
• Protein ID: WP_000650107.1
• Coordinates: 3173926..3174321 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV65_RS15475 (3169790)	3169790..3172048	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
LHV65_RS15480 (3172186)	3172186..3173793	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
LHV65_RS15485 (3173926)	3173926..3174321	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LHV65_RS15490 (3174323)	3174323..3174619	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LHV65_RS15495 (3174824)	3174824..3175306	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
LHV65_RS15500 (3175359)	3175359..3175751	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LHV65_RS15505 (3175903)	3175903..3176562	+	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
LHV65_RS15510 (3176559)	3176559..3177908	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LHV65_RS15515 (3177954)	3177954..3178286	-	333	WP_000917684.1	DUF2645 family protein	-
LHV65_RS15520 (3178605)	3178605..3179186	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LHV65_RS15525 (3179217)	3179217..3179531	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T220057 WP_000415584.1 NZ_CP084898:c3174619-3174323 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T220057 NZ_CP116925:c596367-595903 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT220057 WP_000650107.1 NZ_CP084898:c3174321-3173926 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT220057 NZ_CP116925:c596702-596367 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65