Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3239804..3240531 Replicon chromosome
Accession NZ_CP084895
Organism Escherichia coli strain g74

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LHV63_RS15800 Protein ID WP_000550189.1
Coordinates 3240217..3240531 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LHV63_RS15795 Protein ID WP_000560266.1
Coordinates 3239804..3240220 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV63_RS15785 (3234964) 3234964..3237315 + 2352 WP_000695487.1 alpha-glucosidase -
LHV63_RS15790 (3237741) 3237741..3239759 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LHV63_RS15795 (3239804) 3239804..3240220 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LHV63_RS15800 (3240217) 3240217..3240531 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LHV63_RS15805 (3240815) 3240815..3241951 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LHV63_RS15810 (3242036) 3242036..3242539 + 504 WP_001333820.1 M48 family metallopeptidase -
LHV63_RS15815 (3242616) 3242616..3243308 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
LHV63_RS15820 (3243387) 3243387..3244373 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T219929 WP_000550189.1 NZ_CP084895:c3240531-3240217 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T219929 NZ_CP116900:c884015-883842 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT219929 WP_000560266.1 NZ_CP084895:c3240220-3239804 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT219929 NZ_CP116900:c884339-884043 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References