Detailed information of TA system TA219837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2094178..2094680
• Replicon: chromosome
• Accession: NZ_CP084893
• Organism: Escherichia coli strain g94

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: LHV66_RS10435
• Protein ID: WP_000767829.1
• Coordinates: 2094178..2094432 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LHV66_RS10440
• Protein ID: WP_001259255.1
• Coordinates: 2094429..2094680 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LHV66_RS10405 (2089193)	2089193..2089420	-	228	WP_000234896.1	sulfurtransferase TusA	-
LHV66_RS10410 (2089434)	2089434..2090492	-	1059	WP_000492339.1	thiosulfate utilization transporter TsuA/YeeE	-
LHV66_RS10415 (2090671)	2090671..2092029	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
LHV66_RS10420 (2092019)	2092019..2092081	-	63	WP_010723108.1	membrane protein YoeI	-
LHV66_RS10425 (2092296)	2092296..2093225	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
LHV66_RS10430 (2093271)	2093271..2094095	-	825	WP_000754737.1	SDR family oxidoreductase	-
LHV66_RS10435 (2094178)	2094178..2094432	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LHV66_RS10440 (2094429)	2094429..2094680	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LHV66_RS10445 (2094963)	2094963..2095013	+	51	WP_001364200.1	his operon leader peptide	-
LHV66_RS10450 (2095159)	2095159..2096058	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LHV66_RS10455 (2096064)	2096064..2097368	+	1305	WP_000009594.1	histidinol dehydrogenase	-
LHV66_RS10460 (2097365)	2097365..2098435	+	1071	WP_000108941.1	histidinol-phosphate transaminase	-
LHV66_RS10465 (2098435)	2098435..2099502	+	1068	WP_000080105.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T219837 WP_000767829.1 NZ_CP084893:c2094432-2094178 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T219837 NZ_CP116724:c6052029-6051751 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT219837 WP_001259255.1 NZ_CP084893:c2094680-2094429 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT219837 NZ_CP116724:c6051739-6051434 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0