Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3828162..3828889 Replicon chromosome
Accession NZ_CP084879
Organism Shigella flexneri strain CMCC51574

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag LGV73_RS19505 Protein ID WP_000550189.1
Coordinates 3828575..3828889 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LGV73_RS19500 Protein ID WP_000560266.1
Coordinates 3828162..3828578 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV73_RS19490 (3823522) 3823522..3825873 + 2352 WP_000695506.1 alpha-glucosidase -
LGV73_RS19495 (3826099) 3826099..3828117 + 2019 WP_025745256.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
LGV73_RS19500 (3828162) 3828162..3828578 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
LGV73_RS19505 (3828575) 3828575..3828889 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
LGV73_RS19510 (3829174) 3829174..3830310 - 1137 WP_000018658.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
LGV73_RS19515 (3830395) 3830395..3830898 + 504 WP_001295542.1 M48 family metallopeptidase -
LGV73_RS19520 (3830975) 3830975..3831667 + 693 WP_000942537.1 vancomycin high temperature exclusion protein -
LGV73_RS19525 (3831746) 3831746..3832744 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T219793 WP_000550189.1 NZ_CP084879:c3828889-3828575 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T219793 NZ_CP116717:2111007-2111594 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGAGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT219793 WP_000560266.1 NZ_CP084879:c3828578-3828162 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT219793 NZ_CP116717:2111661-2112515 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACATACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAGTTGCTGCATGCGCG
CTACGTCGAGCAGCGTTTCGCCCCCCATGTGCACAGCGGCTTCGCCCTGGTCTTCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGCGGCGGCGAGCACCTGGCGCCCTGGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGACTCCGGCTGGCTCTATCGCGGCTACTACCCGGAGGTGGAGCAGATCGCCAGTATCCTCGACGAGCTGGAAAT
CCGCCACGGCGGGGTGCCGCGTTTCGCCGACAGCGTGGTCCATGACCAGCCCCTGGCGCGCGCCTTCGTCCATCTGCACC
GCTTGCTGGCCGACCACGCCGGCAGCCTGGAAACCCAGACCTGCTGGCGCGAGACGCTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCGGAGGCGCCGAAGGCCGGGTCGGAACCGTTGGCGGTCGCCCGCGCCCGCGAGTTGCTGGCGGCGCGTTT
GCTCGACCCGCCGTCGCTGGAAGAACTGGCGGCTGCGGTGAATCTGTCGCCGTTCCACTTCGCCCGGGTGTTCCGCCGCG
CCACCGGGATGCCGCCGCATGCCTGGCTCCGGCAACGGCGCCTGGAACAGGCCAGGGCGCTGCTCAAGAATGGCCGGGCG
CCGCTGGATGTCGCCCTGGAACTGGGCTTCGCCGACCAGAGCCATCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
GCCGGGCGAGTACCGCGCGGCCTGTGCGCGCTCGTCCTTGACGATGTGCGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References