Detailed information of TA system TA219790

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2722863..2723088
• Replicon: chromosome
• Accession: NZ_CP084879
• Organism: Shigella flexneri strain CMCC51574

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: LGV73_RS14120
• Protein ID: WP_000813254.1
• Coordinates: 2722863..2723018 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2723030..2723088 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGV73_RS14065	2718296..2718993	+	698	WP_227804319.1	IS1 family transposase	-
LGV73_RS14070	2719005..2719196	-	192	Protein_2750	helix-turn-helix domain-containing protein	-
LGV73_RS14075	2719295..2719510	-	216	WP_000839572.1	class II holin family protein	-
LGV73_RS14100	2720306..2720994	-	689	Protein_2752	bacteriophage antitermination protein Q	-
LGV73_RS14105	2720991..2721356	-	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
LGV73_RS14110	2721357..2722415	-	1059	WP_001265248.1	DUF968 domain-containing protein	-
LGV73_RS14115	2722417..2722695	-	279	WP_011069426.1	hypothetical protein	-
LGV73_RS14120	2722863..2723018	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2723030..2723088	+	59	-	-	Antitoxin
LGV73_RS14125	2723670..2724086	-	417	WP_005069274.1	hypothetical protein	-
LGV73_RS14130	2724113..2724253	+	141	Protein_2758	DUF4224 domain-containing protein	-
LGV73_RS14135	2724253..2725303	+	1051	Protein_2759	tyrosine-type recombinase/integrase	-
LGV73_RS14145	2725514..2726311	+	798	WP_001325918.1	DgsA anti-repressor MtfA	-
LGV73_RS14155	2726649..2727911	+	1263	Protein_2761	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2700682..2761720	61038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T219790 WP_000813254.1 NZ_CP084879:c2723018-2722863 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T219790 NZ_CP116715:c5609160-5608978 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT219790 NZ_CP084879:2723030-2723088 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin