Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1154401..1155019 Replicon chromosome
Accession NZ_CP084879
Organism Shigella flexneri strain CMCC51574

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LGV73_RS05850 Protein ID WP_001291435.1
Coordinates 1154401..1154619 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag LGV73_RS05855 Protein ID WP_000344797.1
Coordinates 1154645..1155019 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV73_RS05820 (1150293) 1150293..1150604 - 312 WP_000409911.1 MGMT family protein -
LGV73_RS05830 (1150983) 1150983..1151336 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LGV73_RS05835 (1151378) 1151378..1152928 - 1551 WP_005106783.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LGV73_RS05840 (1153092) 1153092..1153562 - 471 WP_000136192.1 YlaC family protein -
LGV73_RS05845 (1153678) 1153678..1154229 - 552 WP_272606642.1 maltose O-acetyltransferase -
LGV73_RS05850 (1154401) 1154401..1154619 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LGV73_RS05855 (1154645) 1154645..1155019 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
LGV73_RS05860 (1155565) 1155565..1158714 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
LGV73_RS05865 (1158737) 1158737..1159930 - 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1148935..1150263 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T219777 WP_001291435.1 NZ_CP084879:c1154619-1154401 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T219777 NZ_CP116702:288108-288527 [Agrobacterium fabacearum]
ATGATCTTGCTTGACACCAACGTCATATCCGAACCTTGGAAACCGGTTCCGGACGAGGCGGTGATTGCCTGGTTGGACGC
CCAAGCTGTCGAAACGCTGTTCATCTCCGCAATAACGATTGCGGAACTTCGCTTCGGAATTGCTGCTATGCCTTCAGGCA
GACGGCAAACTATCCTCCGCGACCGTCTCGAGGGCGAGGTGCTGCCACACTTTTCCGGCCGCATCCTGTCCTTCGATCTG
ACTACTTCTCAGTTCTACTCTGAACTGATGGCACGCGCTCGAGCGTCTGGGAAAGCAATCGGCACGGCCGATGGCTACAT
TGCAGCCACAGCGGCAGCAAATGGCCTCACGATCTCAACTCGCGATACAAGCCCTTTCGAGGCTGCCGGGGTGAAAGTGA
TCAATCCCTGGTCCAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT219777 WP_000344797.1 NZ_CP084879:c1155019-1154645 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT219777 NZ_CP116702:287857-288111 [Agrobacterium fabacearum]
ATGCCGGCAGTCACCATTCGTAACCTTTCTGAAGAAACACATCGCGCTCTGAGGGTGCGAGCGGCTCATCATGGCCGGAG
CACGGAGGCCGAGATTCGTGACATTATTGAAGCGGCTGTTCGTCCTCCGGAACGTATTCAGCTCGGGTCGTTGCTAGCGG
CCATTGGTCGCGACGCCGAGCTTTCCAACAGCGACGTTGAAGCTCTTCAGAAAAGTCGCGACAAGACACCTGCTAAGCCG
ATGACCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References