Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1117804..1118257 Replicon chromosome
Accession NZ_CP084879
Organism Shigella flexneri strain CMCC51574

Toxin (Protein)


Gene name itaT Uniprot ID A0A822PL35
Locus tag LGV73_RS05655 Protein ID WP_005053055.1
Coordinates 1117804..1117962 (-) Length 53 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag LGV73_RS05660 Protein ID WP_005053053.1
Coordinates 1117946..1118257 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV73_RS05640 (1114363) 1114363..1116516 - 2154 WP_005060811.1 SEL1-like repeat protein -
LGV73_RS05645 (1116667) 1116667..1117364 + 698 WP_232046043.1 IS1 family transposase -
LGV73_RS05650 (1117412) 1117412..1117753 - 342 WP_005125115.1 GNAT family N-acetyltransferase -
LGV73_RS05655 (1117804) 1117804..1117962 - 159 WP_005053055.1 hypothetical protein Toxin
LGV73_RS05660 (1117946) 1117946..1118257 - 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
LGV73_RS05665 (1118442) 1118442..1119332 - 891 WP_000971346.1 heme o synthase -
LGV73_RS05670 (1119344) 1119344..1119673 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LGV73_RS05675 (1119673) 1119673..1120287 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LGV73_RS05680 (1120277) 1120277..1122267 - 1991 Protein_1116 cytochrome o ubiquinol oxidase subunit I -
LGV73_RS05685 (1122289) 1122289..1122786 - 498 Protein_1117 COX aromatic rich motif-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1116861..1117364 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5914.82 Da        Isoelectric Point: 6.9172

>T219776 WP_005053055.1 NZ_CP084879:c1117962-1117804 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRATRIAKEKCA

Download         Length: 159 bp

>T219776 NZ_CP116702:c174582-174199 [Agrobacterium fabacearum]
GTGATCCTGGTAGACACCTCGATCTGGATAAATCATTTTCGATACGATGACTCAGAACTACGAAAAATCATCAATGATGA
CCAGTTGCTCTGCCACCCATTCGTTGTGGGCGAATTGGCGCTCGGCAGCTTAAGAGAGAGGGCTGCAGTTTTAGAATTCC
TAGCAGCTCAACGCGAGGCGCTAATCGCGACCCATGCAGAAGTAATGACGATCATTGATCGTCATTCGATATTCAGCATG
GGGATTGGTTACACAGATGCGCATTTACTTACTTCAACGTTGCTCGACCGGCGATCAAGCTTGTGGACAAGAGATAAGCG
CCTAGCGGCCGCAGCACAAAAAGTTGGTGTGGCACTTTACACGCACGCGCAAGCCTCTCGTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT219776 WP_005053053.1 NZ_CP084879:c1118257-1117946 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT219776 NZ_CP116702:c174779-174579 [Agrobacterium fabacearum]
ATGCGATCAACCATAAATATCGACGACAATCTTATGGAACGGGCCAAGTCCCTCACGGGCACCAAGGAAACAGCAGCTCT
CGTGCGGCAAGCGCTGGAGACACTCGTGCGTGTGGAATCCGGCAAACGGCTGATCGCGCTTGGCGGCTCTATGCCTGAGG
CTGAAGCGTCGCCTCGCCGACGGAGCGACGTGGCAAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PL35

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References