Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1004404..1005098 Replicon chromosome
Accession NZ_CP084879
Organism Shigella flexneri strain CMCC51574

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag LGV73_RS04965 Protein ID WP_001263491.1
Coordinates 1004700..1005098 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag LGV73_RS04960 Protein ID WP_000554759.1
Coordinates 1004404..1004697 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV73_RS04940 (1000043) 1000043..1000540 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
LGV73_RS04945 (1000757) 1000757..1002469 - 1713 Protein_970 flagellar biosynthesis protein FlhA -
LGV73_RS04950 (1002441) 1002441..1003226 + 786 WP_000207561.1 putative lateral flagellar export/assembly protein LafU -
LGV73_RS04955 (1003297) 1003297..1004352 + 1056 WP_001226172.1 DNA polymerase IV -
LGV73_RS04960 (1004404) 1004404..1004697 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LGV73_RS04965 (1004700) 1004700..1005098 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LGV73_RS04970 (1005108) 1005108..1005560 + 453 WP_001059861.1 GNAT family N-acetyltransferase -
LGV73_RS04975 (1005806) 1005806..1006849 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
LGV73_RS04980 (1006965) 1006965..1007525 + 561 Protein_977 peptide chain release factor H -
LGV73_RS04985 (1007582) 1007582..1009039 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
LGV73_RS04990 (1009301) 1009301..1009759 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / gtrII / gtrB / gtrA / gtrB 924739..1078639 153900
- inside Prophage - gtrII / gtrB / gtrA / gtrB 998222..1083232 85010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T219775 WP_001263491.1 NZ_CP084879:1004700-1005098 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T219775 NZ_CP116702:132733-133026 [Agrobacterium fabacearum]
ATGCCATTTAGCCTATCTGTCCAAGCGGAAGAGGATATTATTTCCATTGCAGAAGAAGGCATCCGCATCTTCGGAGCACT
AGTCGCCAGACAATATCACGATGAGCTTTTTGCCTTGCTCGAACTCATCGCCACCAACCCAAGAATGGCACGCGAAAGCC
ATGAGATTTCTCCTCCTGTCAGGATCCATCCTTTCAAGGCTCACCTTGTCGTGTACCGCGTCATTGAAGACGGGAGCGTA
TTCGTCATCCGCATACGTCACGGCCATGAAGATTGGGCGGGAGATTCTTTCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT219775 WP_000554759.1 NZ_CP084879:1004404-1004697 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT219775 NZ_CP116702:132479-132733 [Agrobacterium fabacearum]
ATGGCGACGATGAATGTATCCCTGCCAGACCCCATGAAAGATTGGGTTGAAGCACAGGCCAAGACTGGTCGATACTCGAA
TGCGAGCGATTATGTCCGGGACCTGATCCGCAGAGACCAAGCGCGTACGGACAAGATTGCCGAGATGCAGCGATTTGTCG
ACGACGGGATCAGAAGCGGAGTTGGTAGCCGTTCAACGGAAGAGTTGTTTGCTTTAGCCACTTCTCGAGCTGACATCAGC
CGCGGACGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References