Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1783137..1783797 Replicon chromosome
Accession NZ_CP084874
Organism Klebsiella africana strain 200023

Toxin (Protein)


Gene name higB Uniprot ID A0A8B6IX92
Locus tag LGL98_RS08590 Protein ID WP_008804168.1
Coordinates 1783444..1783797 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7U2ZUJ7
Locus tag LGL98_RS08585 Protein ID WP_061153979.1
Coordinates 1783137..1783439 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGL98_RS08535 (LGL98_08535) 1778195..1778752 + 558 Protein_1668 hypothetical protein -
LGL98_RS08540 (LGL98_08540) 1779111..1779572 + 462 WP_136032146.1 hypothetical protein -
LGL98_RS08545 (LGL98_08545) 1779569..1779775 + 207 WP_136032094.1 helix-turn-helix transcriptional regulator -
LGL98_RS08550 (LGL98_08550) 1780110..1780367 + 258 WP_000288812.1 antitoxin -
LGL98_RS08555 (LGL98_08555) 1780368..1780700 + 333 WP_040245064.1 type II toxin-antitoxin system PemK/MazF family toxin -
LGL98_RS08570 (LGL98_08570) 1781022..1782458 + 1437 WP_168435312.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LGL98_RS08580 (LGL98_08580) 1782784..1782933 - 150 Protein_1674 antitoxin -
LGL98_RS08585 (LGL98_08585) 1783137..1783439 - 303 WP_061153979.1 XRE family transcriptional regulator Antitoxin
LGL98_RS08590 (LGL98_08590) 1783444..1783797 - 354 WP_008804168.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGL98_RS08595 (LGL98_08595) 1784210..1784668 + 459 WP_136032098.1 hypothetical protein -
LGL98_RS08600 (LGL98_08600) 1784757..1786211 - 1455 WP_136032100.1 AMP nucleosidase -
LGL98_RS08605 (LGL98_08605) 1786342..1786587 - 246 WP_136032102.1 signal transduction protein PmrD -
LGL98_RS08610 (LGL98_08610) 1786858..1788480 + 1623 WP_136032104.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(29-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13528.51 Da        Isoelectric Point: 10.1729

>T219731 WP_008804168.1 NZ_CP084874:c1783797-1783444 [Klebsiella africana]
VWTIKTTDMFDRWFTSLNDIDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRVQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYHEMLAVADREFTNWLNRLKEKE

Download         Length: 354 bp

>T219731 NZ_CP116672:c4770281-4770129 [Xanthomonas hortorum pv. pelargonii]
ATGAAGCGACTGCTGACACTGATGGTGCTAGGCCTGTTTTCGGCTGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
CGGCAAGGACATGCAGGGCGCAGGTGAGAAGGTCGAAAAGAAGGCCGATAATTGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11135.94 Da        Isoelectric Point: 5.1976

>AT219731 WP_061153979.1 NZ_CP084874:c1783439-1783137 [Klebsiella africana]
MGRTLEQLLIDEKPDVVAEAQAIAKDILLNIHLAELREKVQKTQVEMAQDLGIKQPTVAGMEKPGRDLKLSTLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT219731 NZ_CP116672:c4770050-4769916 [Xanthomonas hortorum pv. pelargonii]
ATGAAGCGTGCAATTATGCTGCTGGTGCTGTCGGTGTTTTCGGTCGGTATGTTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAAGTGGAAGACGCTGCGCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B6IX92

Antitoxin

Source ID Structure
AlphaFold DB A0A7U2ZUJ7

References