Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3962480..3963077 Replicon chromosome
Accession NZ_CP084871
Organism Klebsiella pneumoniae strain 04A025

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LGM00_RS19470 Protein ID WP_004142563.1
Coordinates 3962760..3963077 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LGM00_RS19465 Protein ID WP_004142561.1
Coordinates 3962480..3962767 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM00_RS19435 (3958560) 3958560..3958808 + 249 WP_020325243.1 DUF1158 domain-containing protein -
LGM00_RS19440 (3958826) 3958826..3959167 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LGM00_RS19445 (3959198) 3959198..3960313 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
LGM00_RS19450 (3960493) 3960493..3961074 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
LGM00_RS19455 (3961074) 3961074..3961442 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
LGM00_RS19460 (3961562) 3961562..3962215 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LGM00_RS19465 (3962480) 3962480..3962767 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LGM00_RS19470 (3962760) 3962760..3963077 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGM00_RS19475 (3963262) 3963262..3964305 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
LGM00_RS19480 (3964975) 3964975..3965841 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LGM00_RS19485 (3965950) 3965950..3967377 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T219703 WP_004142563.1 NZ_CP084871:c3963077-3962760 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T219703 NZ_CP116600:2090826-2091731 [Burkholderia pseudomallei]
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCGTCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCCAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGGATCGCAGGCGAGCGGCTGGCGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCTGCAGGT
GATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGACGCCTTTTGCCGTCAAT
GCGACGGATCGCGAGGCACACCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT219703 WP_004142561.1 NZ_CP084871:c3962767-3962480 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT219703 NZ_CP116600:2091728-2092582 [Burkholderia pseudomallei]
ATGAGCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCT
GCGGATCCTGGCCGAGTTCTCGCCGCGAGAGCCGGTGCTCGGCGCGCCGGAGCTCTCGAAGCGGCTCGGCATTCCGCGCA
CGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCGGACAAGGATCGCAACTATCGCCTG
GGCGTCGCGGTGCTGCGCCTCGGCTTCGAATACCTGAGCTCGCTCGAGCTGACCGATCTCGGCTTGCCGGTGATCGAAAG
CCTGCGCGATGCGACGGGCTTCACCGCGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAAA
ACGCAGGGAACGCGTTCGGCTCGGTGCGGGTGAACGTCGGCACGCGCCTGCCCGCGCATGCGACGACGCACGGCCACGTG
CTGATGGGCGACCTGTCGCTCGCGCAGTTGCGCGCGTTGTATCCGGAGCGGGCGCTCGAACGCGTGACGAAGGCGACGCC
CGAGACCGTCGACGCGCTCTTCGACGTGATCCGCGACGATGCGCGGCGCGGCTACGGCATCGGCAACTCGGCGTTCGAGC
GCGACATCTCGGTCGTCACGGCGCCCGTGCGCAACGAAGCGAGCCGGATCGTCGCGGTCGTCACGGTGACGGTGCCGCGG
CCGGAGATCGATGCGGCGCTCGTCGCCGAAGGGCTCGTCGAGAAGGTCGTGCGTGCGGCGGCCGAGCTGTCGCGGCGGCT
GAACTATCGGTCCGACGACGAAAACACCTATTTGAAGGCATTGGGATTGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References