Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324239..324825 Replicon chromosome
Accession NZ_CP084871
Organism Klebsiella pneumoniae strain 04A025

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LGM00_RS01510 Protein ID WP_002920800.1
Coordinates 324457..324825 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag LGM00_RS01505 Protein ID WP_004174006.1
Coordinates 324239..324460 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM00_RS01485 (320396) 320396..321322 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LGM00_RS01490 (321319) 321319..322596 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
LGM00_RS01495 (322593) 322593..323360 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LGM00_RS01500 (323362) 323362..324075 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LGM00_RS01505 (324239) 324239..324460 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LGM00_RS01510 (324457) 324457..324825 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LGM00_RS01515 (325098) 325098..326414 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LGM00_RS01520 (326521) 326521..327408 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LGM00_RS01525 (327405) 327405..328250 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LGM00_RS01530 (328252) 328252..329322 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320396..330059 9663


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T219692 WP_002920800.1 NZ_CP084871:324457-324825 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T219692 NZ_CP116581:178411-178599 [Enterococcus durans]
ATGCCCATGACAATTAGAGAGGCAAAAAAATTACTCAAAGAAGCTGGCTTTGTCGAAGTGAAAGGTGGGAAGGGTTCTCA
CAGGAAATTTATTAAAGAGAATTTTCCGCGCCCGGTCATTCTTACCTGTCATTCAAAAGAGTTATCACAACGAGTAGAAA
ATACTGTCCGAAAGGCAGTGGGCTTATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT219692 WP_004174006.1 NZ_CP084871:324239-324460 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT219692 NZ_CP116581:178635-179021 [Enterococcus durans]
ATGAAAAAAGTATATCCAGCTATTTTTGAAAAAGATCCCGTGGGGTATGGTATTTATTTTCCAGATGTGGAGGGTGCAGT
GACTCAAGCTGAAACTATTTTAGAAGGGCTGGAAGTCGCTTCGGATGCTCTTGGCATAATGCTTGGTGATTTGGTAGAGC
GTAATCTTCCTTTACCTGAGCCAAGTACTATGAAGGAAATTTCCTATGATCCTGAGACGGAGTTTGCGACATTAGTGTCA
GTGGAGTTAGATGACTACTTAAAAGATGTAAAACTTGATAAAAAGACGATAAAGATTCCACATTGGCTAAATGTTAGAGC
GACAAATGAAGGCATTAATTTTTCGAAAACGATGACTGAAGCGTTAGCTAAAAAACTGAATATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References