Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1858050..1858647 Replicon chromosome
Accession NZ_CP084870
Organism Klebsiella pneumoniae strain BJ1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LGM01_RS08890 Protein ID WP_004142563.1
Coordinates 1858050..1858367 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LGM01_RS08895 Protein ID WP_004142561.1
Coordinates 1858360..1858647 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM01_RS08875 (1853754) 1853754..1855181 - 1428 WP_004176980.1 MFS transporter -
LGM01_RS08880 (1855290) 1855290..1856156 + 867 WP_004191455.1 helix-turn-helix transcriptional regulator -
LGM01_RS08885 (1856822) 1856822..1857865 + 1044 WP_016946853.1 DUF2157 domain-containing protein -
LGM01_RS08890 (1858050) 1858050..1858367 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGM01_RS08895 (1858360) 1858360..1858647 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LGM01_RS08900 (1858912) 1858912..1859565 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LGM01_RS08905 (1859685) 1859685..1860053 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LGM01_RS08910 (1860053) 1860053..1860634 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LGM01_RS08915 (1860814) 1860814..1861929 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LGM01_RS08920 (1861960) 1861960..1862301 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LGM01_RS08925 (1862319) 1862319..1862567 - 249 WP_032445695.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T219680 WP_004142563.1 NZ_CP084870:1858050-1858367 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T219680 NZ_CP084870:1858050-1858367 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAAGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT219680 WP_004142561.1 NZ_CP084870:1858360-1858647 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT219680 NZ_CP084870:1858360-1858647 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References