Detailed information of TA system TA219673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 101869..102515
• Replicon: chromosome
• Accession: NZ_CP084870
• Organism: Klebsiella pneumoniae strain BJ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A231WWF9
• Locus tag: LGM01_RS00590
• Protein ID: WP_004174016.1
• Coordinates: 102168..102515 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGM01_RS00585
• Protein ID: WP_223367845.1
• Coordinates: 101869..102114 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM01_RS00565 (98075)	98075..98833	-	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-
LGM01_RS00570 (98883)	98883..99713	-	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
LGM01_RS00575 (99764)	99764..100093	-	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
LGM01_RS00580 (100298)	100298..101806	+	1509	WP_019704656.1	glycerol-3-phosphate dehydrogenase	-
LGM01_RS00585 (101869)	101869..102114	-	246	WP_223367845.1	helix-turn-helix transcriptional regulator	Antitoxin
LGM01_RS00590 (102168)	102168..102515	-	348	WP_004174016.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGM01_RS00595 (102691)	102691..105138	-	2448	WP_002920561.1	glycogen phosphorylase	-
LGM01_RS00600 (105156)	105156..106589	-	1434	WP_032445350.1	glycogen synthase GlgA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-66)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13518.55 Da
• Isoelectric Point: 6.2327

>T219673 WP_004174016.1 NZ_CP084870:c102515-102168 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T219673 NZ_CP116571:2828297-2828440 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8717.13 Da
• Isoelectric Point: 8.6165

>AT219673 WP_223367845.1 NZ_CP084870:c102114-101869 [Klebsiella pneumoniae]
IAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALGGELSINVTLPTGKKVSFN
L

Nucleotide

• Length: 246 bp

>AT219673 NZ_CP116571:c2828634-2828372 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A231WWF9