Detailed information of TA system TA219650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57417..58144
• Replicon: plasmid pKp_SA12_1
• Accession: NZ_CP084867
• Organism: Klebsiella pneumoniae strain SA12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LGM09_RS26525
• Protein ID: WP_011251285.1
• Coordinates: 57833..58144 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGM09_RS26520
• Protein ID: WP_011251286.1
• Coordinates: 57417..57836 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM09_RS26490 (LGM09_26490)	52585..53055	-	471	WP_048333570.1	hypothetical protein	-
LGM09_RS26495 (LGM09_26495)	53368..54003	-	636	WP_223171879.1	hypothetical protein	-
LGM09_RS26500 (LGM09_26500)	54422..55042	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LGM09_RS26505 (LGM09_26505)	55063..55851	+	789	WP_040217257.1	hypothetical protein	-
LGM09_RS26510 (LGM09_26510)	55865..56230	+	366	WP_048333448.1	hypothetical protein	-
LGM09_RS26515 (LGM09_26515)	56302..57270	-	969	WP_074428168.1	IS5 family transposase	-
LGM09_RS26520 (LGM09_26520)	57417..57836	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LGM09_RS26525 (LGM09_26525)	57833..58144	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LGM09_RS26530 (LGM09_26530)	58349..58786	-	438	Protein_58	DDE-type integrase/transposase/recombinase	-
LGM09_RS26535 (LGM09_26535)	58921..59618	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LGM09_RS26540 (LGM09_26540)	61004..61654	-	651	WP_068893702.1	DUF1173 family protein	-
LGM09_RS26545 (LGM09_26545)	61687..61953	-	267	WP_223175074.1	DUF1173 family protein	-
LGM09_RS26550 (LGM09_26550)	62131..62625	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..230142	230142
	-	inside	IScluster/Tn	-	-	47296..59618	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T219650 WP_011251285.1 NZ_CP084867:c58144-57833 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T219650 NZ_CP116567:c64329-63988 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGTTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT219650 WP_011251286.1 NZ_CP084867:c57836-57417 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT219650 NZ_CP116567:c64559-64329 [Enterococcus faecalis]
ATGTTAACAACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1