Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3958325..3958922 Replicon chromosome
Accession NZ_CP084852
Organism Klebsiella pneumoniae strain SB2390

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LGM13_RS19375 Protein ID WP_004142563.1
Coordinates 3958605..3958922 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LGM13_RS19370 Protein ID WP_004142561.1
Coordinates 3958325..3958612 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM13_RS19340 (3954405) 3954405..3954653 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LGM13_RS19345 (3954671) 3954671..3955012 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LGM13_RS19350 (3955043) 3955043..3956158 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
LGM13_RS19355 (3956338) 3956338..3956919 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LGM13_RS19360 (3956919) 3956919..3957287 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LGM13_RS19365 (3957407) 3957407..3958060 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LGM13_RS19370 (3958325) 3958325..3958612 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LGM13_RS19375 (3958605) 3958605..3958922 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGM13_RS19380 (3959107) 3959107..3960150 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
LGM13_RS19385 (3960817) 3960817..3961683 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
LGM13_RS19390 (3961792) 3961792..3963219 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T219567 WP_004142563.1 NZ_CP084852:c3958922-3958605 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T219567 NZ_CP116523:479745-480086 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT219567 WP_004142561.1 NZ_CP084852:c3958612-3958325 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT219567 NZ_CP116523:479515-479745 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References