Detailed information of TA system TA219553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 85050..85777
• Replicon: plasmid pKp_SB611_1
• Accession: NZ_CP084844
• Organism: Klebsiella pneumoniae strain SB611

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: LGM15_RS26870
• Protein ID: WP_000558568.1
• Coordinates: 85466..85777 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGM15_RS26865
• Protein ID: WP_000990393.1
• Coordinates: 85050..85469 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGM15_RS26830 (LGM15_26830)	80737..81099	-	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-
LGM15_RS26835 (LGM15_26835)	81147..81500	-	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LGM15_RS26840 (LGM15_26840)	81793..81933	+	141	WP_004118614.1	hypothetical protein	-
LGM15_RS26845 (LGM15_26845)	82062..82409	+	348	WP_223196907.1	Ref family recombination enhancement nuclease	-
LGM15_RS26850 (LGM15_26850)	82833..83492	+	660	WP_000078540.1	hypothetical protein	-
LGM15_RS26855 (LGM15_26855)	83489..83641	+	153	WP_226651217.1	hypothetical protein	-
LGM15_RS26860 (LGM15_26860)	83811..85013	+	1203	WP_194069787.1	phage tail protein	-
LGM15_RS26865 (LGM15_26865)	85050..85469	-	420	WP_000990393.1	helix-turn-helix domain-containing protein	Antitoxin
LGM15_RS26870 (LGM15_26870)	85466..85777	-	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
LGM15_RS26875 (LGM15_26875)	85988..86368	+	381	Protein_99	gluconate permease	-
LGM15_RS26880 (LGM15_26880)	86418..87113	-	696	WP_000370412.1	lactate utilization protein C	-
LGM15_RS26885 (LGM15_26885)	87106..88533	-	1428	WP_000023895.1	LutB/LldF family L-lactate oxidation iron-sulfur protein	-
LGM15_RS26890 (LGM15_26890)	88544..89263	-	720	WP_001102107.1	(Fe-S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / mrkA	1..155131	155131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T219553 WP_000558568.1 NZ_CP084844:c85777-85466 [Klebsiella pneumoniae]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T219553 NZ_CP116496:c109054-108725 [Rickettsia asembonensis]
ATGGTAAATTATATACCTGAAAAAGGTGATATAGTTTGGCTTGATTTTGAACCGCAAAAAGGTAAAGAAATTCAAAAGAC
AAGACCGGCGGTTGTACTTAGCCCTTACAAATATCATCTTAAAAGTGGTCTTGCTTTATTTGCTCCTATTACTAGTCAAA
TAAAAAATTATCCCTTTGAAGTAATAATAGATTTTCAGCAAATAAAGGGAGCGGTATTATGCGATCAAGTTAGATCAATG
GACTTCAAAGCACGTAAAGCAAATAAAATTTTAACATTAAATAAAATACTTATAGATGAAATAATACATAAATTAAAATT
ACTTTTATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15560.74 Da
• Isoelectric Point: 4.3786

>AT219553 WP_000990393.1 NZ_CP084844:c85469-85050 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQYKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT219553 NZ_CP116496:c109284-109048 [Rickettsia asembonensis]
ATGCAAAGTCAAATTAAAAAATGGGGAAATAGTTTAGGTATTAGAATACCTAAACACTTAGCAGGGAAACTACATGTTAG
TGAAGGTACCTTAGTTAATTTAAAATTAGTCAATAATAGTTTAATTATTAGCACAGAAAAATCTGAATTAGATACATTGC
TAGATAATATTACTGACTCTAATCGTCATCATGAATTATTAAGTAATGATAAGACTAAAGGTAATGAAACATGGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1