Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3884615..3885212 Replicon chromosome
Accession NZ_CP084830
Organism Klebsiella pneumoniae strain SB612

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LGM16_RS18960 Protein ID WP_004142563.1
Coordinates 3884895..3885212 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LGM16_RS18955 Protein ID WP_004142561.1
Coordinates 3884615..3884902 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGM16_RS18925 (LGM16_18925) 3880695..3880943 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LGM16_RS18930 (LGM16_18930) 3880961..3881302 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LGM16_RS18935 (LGM16_18935) 3881333..3882448 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LGM16_RS18940 (LGM16_18940) 3882628..3883209 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LGM16_RS18945 (LGM16_18945) 3883209..3883577 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LGM16_RS18950 (LGM16_18950) 3883697..3884350 + 654 WP_019704893.1 oxygen-insensitive NAD(P)H nitroreductase -
LGM16_RS18955 (LGM16_18955) 3884615..3884902 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LGM16_RS18960 (LGM16_18960) 3884895..3885212 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGM16_RS18965 (LGM16_18965) 3885397..3886440 - 1044 WP_019704892.1 DUF2157 domain-containing protein -
LGM16_RS18970 (LGM16_18970) 3887110..3887976 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LGM16_RS18975 (LGM16_18975) 3888085..3889512 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T219523 WP_004142563.1 NZ_CP084830:c3885212-3884895 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T219523 NZ_CP116489:784233-784541 [Haemophilus influenzae]
ATGTCAGAAGAGAAGCCATTAAAAGTGAGCTATTCAAAACAATTTGTAAGAGATTTAACTGATTTAGCTAAACGTTCCCC
CAATGTGTTAATTGGCTCAAAATACATTACTGCAATACACTGTTTATTAAATCGTTTACCTTTACCCGAAAACTACCAAG
ATCACGCATTAGTAGGAGAGTGGAAAGGTTATCGAGATTGCCATATTCAAGGTGATTTGGTACTGATTTACCAATACGTT
ATACAGGATGAATTTGATGAATTGAAATTTTCTCGTTTAAATACACACTCACAAACCGCTTTAAAATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT219523 WP_004142561.1 NZ_CP084830:c3884902-3884615 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT219523 NZ_CP116489:783937-784233 [Haemophilus influenzae]
ATGGCACTTACGAATTCCTCTATCAGCTTTAGAACAGTTGAGAAAACAAAATTAGAAGCTTATCAAGTTATTGAACAATA
TGGACTTACCCCATCACAAGTATTCAATATGTTTTTGGCTCAAATTGCCAAGACTCGCTCTATCCCTGTTGATTTGAATT
ATCTTCGCCCAAATAAAGAAACCCTAGCAGCTATTGATGAATTAGATAGTGGTAACGCAGAGAGCTTCTTTATTGAAGCT
AGTGAAAATTATTCTGCCGAAGAATTTACAAAACGCATTCTTAATGGTGGTCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References