Detailed information of TA system TA219331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1774797..1775394
• Replicon: chromosome
• Accession: NZ_CP084767
• Organism: Klebsiella variicola subsp. tropica strain CDC4241-71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: LGN97_RS08375
• Protein ID: WP_012542526.1
• Coordinates: 1774797..1775114 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGN97_RS08380
• Protein ID: WP_074386636.1
• Coordinates: 1775107..1775394 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN97_RS08360 (LGN97_08360)	1770947..1772374	-	1428	WP_074386638.1	MFS transporter	-
LGN97_RS08365 (LGN97_08365)	1772483..1773349	+	867	WP_136086451.1	helix-turn-helix transcriptional regulator	-
LGN97_RS08370 (LGN97_08370)	1773570..1774613	+	1044	WP_100729357.1	DUF2157 domain-containing protein	-
LGN97_RS08375 (LGN97_08375)	1774797..1775114	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGN97_RS08380 (LGN97_08380)	1775107..1775394	+	288	WP_074386636.1	helix-turn-helix transcriptional regulator	Antitoxin
LGN97_RS08385 (LGN97_08385)	1775651..1776304	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LGN97_RS08390 (LGN97_08390)	1776424..1776792	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
LGN97_RS08395 (LGN97_08395)	1776792..1777373	-	582	WP_046881940.1	TetR/AcrR family transcriptional regulator	-
LGN97_RS08400 (LGN97_08400)	1777553..1778668	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
LGN97_RS08405 (LGN97_08405)	1778699..1779040	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LGN97_RS08410 (LGN97_08410)	1779057..1779305	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T219331 WP_012542526.1 NZ_CP084767:1774797-1775114 [Klebsiella variicola subsp. tropica]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T219331 NZ_CP116303:1776396-1776653 [Tenacibaculum finnmarkense]
ATGAAGTATACATTCGTTGACGAGTCTTGGGAAGATTATTTGTATTGGCAAAAAACAGACAAAAAAAAGTTAAAAAAAAT
AAATGAACTTCTAAAAGACATTGCTCGAAATCCTTTTGATGGGATTGGAAAACCAGAAGCACTAAAACATAAATACGCAG
GATTTTGGTCAAGACGAATTGATAGTGAGCATAGATTGATTTACCAATATCGAGAAGGAGAAATCTTAATTGCCAAATGT
AGATTCCATTATAACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10801.48 Da
• Isoelectric Point: 10.2212

>AT219331 WP_074386636.1 NZ_CP084767:1775107-1775394 [Klebsiella variicola subsp. tropica]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT219331 NZ_CP116303:1776140-1776394 [Tenacibaculum finnmarkense]
ATGCAAATAACAACTGTTTCTGACTTCAGAAAAGATATTAAAAGTTACTTAGACAGAGTTATCAAAGACTTTGAAACATT
AATAATAAATCGCGGAAAAGATTCTGGAATTGTAGTTATGTCATTGCAGGAATACAATTCTTTAATGGCTACAAATCATG
AATTATCTTCACGAAAAAATGAATTAAGATTAGACTCTGCAATTGACAAACTAAAAAACGGAATGACCTTAAACAAAGAC
CTAATCGAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2