Detailed information of TA system TA219322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3996677..3997274
• Replicon: chromosome
• Accession: NZ_CP084766
• Organism: Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: LGN95_RS19380
• Protein ID: WP_012542526.1
• Coordinates: 3996957..3997274 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LGN95_RS19375
• Protein ID: WP_012542525.1
• Coordinates: 3996677..3996964 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LGN95_RS19345 (LGN95_19345)	3992587..3992835	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
LGN95_RS19350 (LGN95_19350)	3992852..3993193	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LGN95_RS19355 (LGN95_19355)	3993224..3994339	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
LGN95_RS19360 (LGN95_19360)	3994519..3995100	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
LGN95_RS19365 (LGN95_19365)	3995100..3995468	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
LGN95_RS19370 (LGN95_19370)	3995588..3996241	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
LGN95_RS19375 (LGN95_19375)	3996677..3996964	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
LGN95_RS19380 (LGN95_19380)	3996957..3997274	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LGN95_RS19385 (LGN95_19385)	3997459..3998502	-	1044	WP_064170485.1	DUF2157 domain-containing protein	-
LGN95_RS19390 (LGN95_19390)	3999020..3999886	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
LGN95_RS19395 (LGN95_19395)	3999995..4001422	+	1428	WP_012968758.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T219322 WP_012542526.1 NZ_CP084766:c3997274-3996957 [Klebsiella variicola subsp. variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T219322 NZ_CP116266:c6822-6553 [Aeromonas salmonicida subsp. salmonicida]
TTGGCTTGGAAAGTTGAATTGACCGATACCGCCAAGAAGCAATTGGCTAGGCTGGATAAGACCCAATCGCAGAGGATCAC
AAAATATCTGCGGCGGATTATGATGCTTGAAAATCCGAGGGATGCTGGCAAAGCTCTAACCGGTAATTTACGAACCTACT
GGCGTTATCGCGTGGGGGATTATCGGGTTGTGTGTGACATAAGAGATAACGATCTTGTCATTGTTGCTGTGATAATTGGG
CATCGCAGTGAAGTATATGGTGGAAGTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT219322 WP_012542525.1 NZ_CP084766:c3996964-3996677 [Klebsiella variicola subsp. variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT219322 NZ_CP116266:c7030-6806 [Aeromonas salmonicida subsp. salmonicida]
ATGTTAGCGATACGCTTGCCTTCGGAGATCGAAGAGCGTTTGGCAAATTTGGCGCAAGAGACGGGAAGAACGAAGACCTT
CTATGCACGGGAGGCCATCCTGACCCATCTGGAAGAGTTGGAAGATTACTATCTGGCTGAGAGGGTCATGGAGAATGTTC
GCAAGGGCGAAGAGCGCCTGTTCTCATTGGATGACGTGGAGCGCGATCTTGGCTTGGAAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2