Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 345280..345926 Replicon chromosome
Accession NZ_CP084766
Organism Klebsiella variicola subsp. variicola strain 01A065

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag LGN95_RS01565 Protein ID WP_004174016.1
Coordinates 345280..345627 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag LGN95_RS01570 Protein ID WP_008806992.1
Coordinates 345627..345926 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGN95_RS01555 (LGN95_01555) 341167..342600 + 1434 WP_004202133.1 glycogen synthase GlgA -
LGN95_RS01560 (LGN95_01560) 342618..345065 + 2448 WP_008806990.1 glycogen phosphorylase -
LGN95_RS01565 (LGN95_01565) 345280..345627 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LGN95_RS01570 (LGN95_01570) 345627..345926 + 300 WP_008806992.1 XRE family transcriptional regulator Antitoxin
LGN95_RS01575 (LGN95_01575) 345989..347497 - 1509 WP_008806993.1 glycerol-3-phosphate dehydrogenase -
LGN95_RS01580 (LGN95_01580) 347702..348031 + 330 WP_004202138.1 thiosulfate sulfurtransferase GlpE -
LGN95_RS01585 (LGN95_01585) 348082..348912 + 831 WP_008806994.1 rhomboid family intramembrane serine protease GlpG -
LGN95_RS01590 (LGN95_01590) 348962..349720 + 759 WP_008806995.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T219308 WP_004174016.1 NZ_CP084766:345280-345627 [Klebsiella variicola subsp. variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T219308 NZ_CP116249:3686532-3686750 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT219308 WP_008806992.1 NZ_CP084766:345627-345926 [Klebsiella variicola subsp. variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT219308 NZ_CP116249:3686130-3686504 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References